10.5061/DRYAD.4MP00
Gardner, Andrew G.
Rhodes College
Sessa, Emily B.
University of Florida
Michener, Pryce
Rhodes College
Johnson, Eden
Rhodes College
Shepherd, Kelly A.
Howarth, Dianella G.
Jabaily, Rachel S.
Rhodes College
Data from: Utilizing next-generation sequencing to resolve the backbone of
the Core Goodeniaceae and inform future taxonomic and floral form studies
Dryad
dataset
2016
Scaevola
Backbone topology
Selliera
Verreauxia
nuclear ribosomal repeat
Velleia
Goodeniaceae
Coopernookia
Goodenia
Diaspasis
2016-10-19T00:00:00Z
2016-10-19T00:00:00Z
en
https://doi.org/10.1016/j.ympev.2015.10.003
1950265 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Though considerable progress has been made in inferring phylogenetic
relationships of many plant lineages, deep unresolved nodes remain a
common problem that can impact downstream efforts, including taxonomic
decision-making and character reconstruction. The Core Goodeniaceae is a
group affected by this issue: data from the plastid regions trnL-trnF and
matK have been insufficient to generate adequate support at key nodes
along the backbone of the phylogeny. We performed genome skimming for 24
taxa representing major clades within Core Goodeniaceae. The plastome
coding regions (CDS) and nuclear ribosomal repeats (NRR) were assembled
and complemented with additional accessions sequenced for nuclear G3PDH
and plastid trnL-trnF and matk. The CDS, NRR, and G3PDH alignments were
analyzed independently and topology tests were used to detect the
alignments’ ability to reject alternative topologies. The CDS, NRR, and
G3PDH alignments independently supported a Brunonia (Scaevola s.l.
(Coopernookia (Goodenia s.l.))) backbone topology, but within Goodenia
s.l., the strongly-supported plastome topology (Goodenia A (Goodenia B
(Velleia + Goodenia C))) contrasts with the poorly supported nuclear
topology ((Goodenia A + Goodenia B) (Velleia + Goodenia C)). A fully
resolved and maximally supported topology for Core Goodeniaceae was
recovered from the plastome CDS, and there is excellent support for most
of the major clades and relationships among them in all alignments. The
composition of these seven major clades renders many of the current
taxonomic divisions non-monophyletic, prompting us to suggest that
Goodenia may be split into several segregate genera.
NGS resolution of Core Goodeniaceae data filesSee "Dryad
Description" file inside zip folder.NGS resolution of Core
Goodeniaceae.zip
Pacific Islands
Australia