10.5061/DRYAD.4G1H2
Benazeraf, Bertrand
Beaupeux, Mathias
McGill University
Tcherknookov, Martin
McGill University
Wallingford, Allison
Children's Hospital of Los Angeles
Salisbury, Tasha
Children's Hospital of Los Angeles
Shirtz, Amelia
Children's Hospital of Los Angeles
Shirtz, Andrew
Northern Michigan University
Huss, David
Children's Hospital of Los Angeles
Pourquie, Olivier
University of Strasbourg
Francois, Paul
McGill University
Lansford, Rusty
Children's Hospital of Los Angeles
Data from: Multi-scale quantification of tissue behavior during amniote
embryo axis elongation
Dryad
dataset
2017
axis elongation
Morphogenesis
multi-tissue
Transgenic quail embryo
Vertebrate embryo
time-lapse imaging
National Science Foundation
https://ror.org/021nxhr62
Human Frontier Science Program RGP0051
2017-09-05T13:44:24Z
2017-09-05T13:44:24Z
en
https://doi.org/10.1242/dev.150557
1595132421 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Embryonic axis elongation is a complex multi-tissue morphogenetic process
responsible for the formation of the posterior part of the amniote body.
How movements and growth are coordinated between the different posterior
tissues (e.g. neural tube, axial and paraxial mesoderm, lateral plate,
ectoderm, endoderm) to drive axis morphogenesis remain largely unknown.
Here, we use quail embryos to quantify cell behavior and tissue movements
during elongation. We quantify the tissue-specific contribution to axis
elongation by using 3D volumetric techniques, then quantify
tissue-specific parameters such as cell density and proliferation. To
study cell behavior at a multi-tissue scale we used high-resolution 4D
imaging of transgenic quail embryos expressing fluorescent proteins. We
developed specific tracking and image analysis techniques to analyze cell
motion and compute tissue deformations in 4D. This analysis reveals
extensive sliding between tissues during axis extension. Further
quantification of tissue tectonics showed patterns of rotations,
contractions and expansions, which are coherent with the multi-tissue
behavior observed previously. Our approach defines a quantitative and
multiscale method to analyze the coordination between tissue behaviors
during early vertebrate embryo morphogenetic events.
HH10 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. time lapse ventral viewtime
lapse of a stage HH10 PGK1:H2B-chFP embryo ventral view 20x objective on a
Zeiss 780 confocal microscope and Zen 2011 black edition 64 bit
software.Fig 5 and Fig 6stage 10 ventral view.lsmHH10 PGK1:H2B-chFP Tiled
Z Stack 20x.lsm. time lapse dorsal viewtime lapse Dorsal view of a
HH11PGK1:H2B-chFP embryo using a 20x objective on a Zeiss 780 confocal
microscope and Zen 2011 black edition 64 bit software.Fig 5 and Fig 6stage
10 dorsal view.lsm