10.5061/DRYAD.483HR
Etges, William J.
University of Arkansas System
de Oliveira, Cassia
University of Arkansas System
Rajpurohit, Subhash
University of Nevada Reno
Gibbs, Allen G.
University of Nevada Reno
Data from: Preadult life history variation determines adult transcriptome
expression
Dryad
dataset
2015
Stenocereus gummosus
life cycle
Candida valida
Starmera amethionina
Erwinia cacticida
Candida sonorensis
Sporopachydermia cereana
Drosophila mojavensis
Stenocereus thurberi
Microarrays
Dipodascus starmeri
Pichia cactophila
Life History Evolution
Cactus
Development time
Pichia mexicana
Holocene
2015-12-02T21:00:42Z
2015-12-02T21:00:42Z
en
https://doi.org/10.1111/mec.13505
184701 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Preadult determinants of adult fitness and behavior have been documented
in a variety of organisms with complex life cycles, but little is known
about expression patterns of genes underlying these adult traits. We
explored the effects of differences in egg to adult development time on
adult transcriptome and cuticular hydrocarbon variation in order to
understand the nature of the genetic correlation between preadult
development time and premating isolation between populations of Drosophila
mojavensis reared in different host cactus environments. Transcriptome
variation was analyzed separately in flies reared on each host and
revealed that hundreds of genes in adults were differentially expressed
(FDR P < 0.05) due to development time differences. For flies
reared on pitaya agria cactus, longer preadult development times caused
increased expression of genes in adults enriched for ribosome production,
protein metabolism, chromatin remodeling, and regulation of alternate
splicing and transcription. Baja California flies reared on organ pipe
cactus showed fewer differentially expressed genes in adults due to longer
preadult development time, but these were enriched for ATP synthesis and
the TCA cycle. Mainland flies reared on organ pipe cactus with shorter
development times showed increased transcription of genes enriched for
mitochondria and energy production, protein synthesis, and glucose
metabolism: adults with longer development times had increased expression
of genes enriched for adult life span, cuticle proteins and ion binding,
although most differentially expressed genes were unannotated. Differences
due to population, sex, mating status, and their interactions were also
assessed. Adult cuticular hydrocarbon profiles also showed shifts due to
egg to adult development time, and were influenced by population and
mating status. These results help to explain why preadult life history
variation determines subsequent expression of the adult transcriptome
along with traits involved with reproductive isolation and revealed
previously undocumented connections between genetic and environmental
influences over the entire life cycle in this desert insect.
Etges_etal_2015_MEC_CHCdataCuticular hydrocarbon amounts for male and
female Drosophila mojavensis reared on fermenting agria (Stenocereus
gummosus) or organ pipe (S. thurberi) cactus tissues. Samples are based on
mature adults grouped by day of egg to adult development time. Two
populations were studied, LB, from Las Bocas, Sonora, and PP, from Punta
Prieta, Baja California. Hydrocarbon types and chainlengths are described
in Etges and Jackson (2001).
Southwestern U.S.
Northwestern Mexico