10.5061/DRYAD.450C2V7
Morris, Matthew Richard John
Bowles, Ella
University of Calgary
Allen, Brandon Eugene
University of Calgary
Jamniczky, Heather A.
University of Calgary
Rogers, Sean M.
University of Calgary
Data from: Contemporary ancestor? Adaptive divergence from standing
genetic variation in Pacific marine threespine stickleback
Dryad
dataset
2018
Evolutionary genomics
Next-generation sequencing
present
Holocene
Gasterosteus aculeatus
2018-07-10T16:55:57Z
2018-07-10T16:55:57Z
en
https://doi.org/10.1186/s12862-018-1228-8
12217803 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: Populations that have repeatedly colonized novel environments
are useful for studying the role of ecology in adaptive divergence -
particularly if some individuals persist in the ancestral habitat. Such
"contemporary ancestors" can be used to demonstrate the effects
of selection by comparing phenotypic and genetic divergence between the
derived population and their extant ancestors. However, evolution and
demography in these "contemporary ancestors" can complicate
inferences about the source (standing genetic variation, de novo mutation)
and pace of adaptive divergence. Marine threespine stickleback
(Gasterosteus aculeatus) have colonized freshwater environments along the
Pacific coast of North America, but have also persisted in the marine
environment. To what extent are marine stickleback good proxies of the
ancestral condition? Results: We sequenced >5800 variant loci in
over 250 marine stickleback from eight locations extending from Alaska to
California, and phenotyped them for platedness and body shape. Pairwise
FST varied from 0.02 to 0.18. Stickleback were divided into five genetic
clusters, with a single cluster comprising stickleback from Washington to
Alaska. Plate number, Eda, body shape, and candidate loci showed evidence
of being under selection in the marine environment. Comparisons to a
freshwater population demonstrated that candidate loci for freshwater
adaptation varied depending on the choice of marine populations.
Conclusions: Marine stickleback are structured into phenotypically and
genetically distinct populations that have been evolving as freshwater
stickleback evolved. This variation complicates their usefulness as
proxies of the ancestors of freshwater populations. Lessons from
stickleback may be applied to other "contemporary
ancestor"-derived population studies.
Marine stickleback structure fileStacks output as a Structure file for the
"marine sites" data setbatch_1.struAdegenet-recognized clusters
structure fileStacks output as a Structure file, for Adegenet-recognized
clustersadegenet_batch_1.struMarine-freshwater structure fileStacks output
as a Structure file for the marine-freshwater data
setmarinefw_batch_1.struMorphological data setAmira output with
coordinates for 3D landmarks, used in MorphoJ
analysis.landmark_summary_withAK.txtPlate counts and EdaBasic information
on each fish, including standard length, sum of plates on left and right
side, sex, Eda genotype, and which part of the study it was used
in.plates.xlsx
Alaska to California
Pacific coast of North America