10.5061/DRYAD.40494
Connally, Noah
Yale University
Anderson, Christopher P.
Yale University
Bolton, Jules E.
Bolton, Edward W.
Yale University
Buss, Leo W.
Yale University
Data from: The selective myosin II inhibitor blebbistatin reversibly
eliminates gastrovascular flow and stolon tip pulsations in the colonial
hydroid Podocoryna carnea
Dryad
dataset
2016
hydrorhiza
stolon
collective cell movement
Recent
Podocoryna carnea
Cnidaria
Colony
polyp
Hydrozoa
latrunculin
Holocene
blebbistatin
2016-11-11T00:00:00Z
2016-11-11T00:00:00Z
en
https://doi.org/10.1371/journal.pone.0143564
23116870392 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Blebbistatin reversibly disrupted both stolon tip pulsations and
gastrovascular flow in the colonial hydroid Podocoryna carnea. Epithelial
longitudinal muscles of polyps were unaffected by blebbistatin, as polyps
contracted when challenged with a pulse of KCl. Latrunculin B, which
sequesters G actin preventing F actin assembly, caused stolons to retract,
exposing focal adhesions where the tip epithelial cells adhere to the
substratum. These results are consistent with earlier suggestions that
non-muscle myosin II provides the motive force for stolon tip pulsations
and further suggest that tip oscillations are functionally coupled to
hydrorhizal axial muscle contraction.
Intact replicate 1_prior to reagentTiff files from time-lapse movie
acquired during blebbistatin treatment of intact stolon tipIntact
replicate 1_after reagentIntact replicate 1_recoverytiff images from
period of film following clearance of blebbistatinIntact replicate 2_prior
to reagentIntact replicate 2_after reagentIntact replicate
2_recoveryIntact replicate 3_prior to reagentIntact replicate 3_after
reagentIntact replicate 3_recoveryintact replicate 4 prior to
reagentintact replicate 4 after reagentintact replicate 4 recoveryIntact
replicate 5 prior to reagentIntact replicate 5 after reagentIntact
replicate 5 recoveryIsolated replicate 2 prior to reagentIsolated
replicate 2 after reagent addedIsolated replicate 2 recoveryIsolated
replicate 3 prior to reagentIsolated replicate 3 after reagentIsolated
replicate 3 recoveryLatrunculin replicate 1 prior to reagentLatrunculin
replicate 1 after reagentLatrunculin replicate 1 recoveryLatrunculin rep
2_fig 7_pt 1Latrunculin rep 2_fig 7_pt 2Latrunculin replicate 3 prior to
reagentLatrunculin replicate 3 after reagentLatrunculin replicate 3
recoveryIsolated rep 1_fig 6_pt 1aIsolated rep 1_fig 6_pt 1bIsolated rep
1_fig 6_pt 2aIsolated rep 1_fig 6_pt 2bIsolated rep 1_fig 6_pt 2cIsolated
rep 1_fig 6_pt 2dIntact rep 6_fig 5_part 1aIntact rep 6_fig 5_part
1bIntact rep 6_fig 5_part 1cIntact rep 6_fig 5_part 1dIntact_rep 6_fig
5_pt 2aIntact_rep 6_fig 5_pt 2bIntact_rep 6_fig 5_pt 2cIntact_rep 6_fig
5_pt 2dIntact_rep 6_fig 5_pt 2eIntact_rep 6_fig 5_pt 2fIntact_rep 6_fig
5_pt 2gIntact_rep 6_fig 5_pt 2hIntact_rep 6_fig 5_pt 2iIntact_rep 6_fig
5_pt 2jFigure 5 time and lengthFigure 6 time and lengthFigure 7 time and
lengthREAD MEKey to all other files
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