10.5061/DRYAD.3T423
Sklenář, František
Charles University
Jurjević, Željko
Zalar, Polona
University of Ljubljana
Frisvad, J. C.
Technical University of Denmark
Visagie, Cobus M.
University of Ottawa
Kolařík, Miroslav
Charles University
Houbraken, Jos
Westerdijk Fungal Biodiversity Institute
Chen, Amanda J.
Yilmaz, Neriman
University of Ottawa
Seifert, Keith A.
University of Ottawa
Coton, Monica
Déniel, Franck
Gunde-Cimerman, Nina
University of Ljubljana
Samson, Robert A.
Westerdijk Fungal Biodiversity Institute
Peterson, Stephen W.
United States Department of Agriculture
Hubka, Vít
Charles University
Data from: Phylogeny of xerophilic aspergilli (subgenus Aspergillus) and
taxonomic revision of section Restricti
Dryad
dataset
2017
indoor fungi
multigene phylogeny
Aspergillus canadensis
food spoilage
Aspergillus destruens
Aspergillus restrictus
Aspergillus tardicrescens
Aspergillus clavatophorus
Aspergillus villosus
Aspergillus glabripes
Aspergillus infrequens
Aspergillus domesticus
Aspergillus magnivesiculatus
Aspergillus pseudogracilis
Aspergillus penicillioides
sick building syndrome
Aspergillus pachycaulis
xerophilic fungi
Aspergillus reticulatus
Aspergillus hordei
Linear discriminant analysis
Aspergillus salinicola
multispecies coalescent model
Eurotium
2017-10-10T21:34:33Z
2017-10-10T21:34:33Z
en
https://doi.org/10.1016/j.simyco.2017.09.002
3913486 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Aspergillus section Restricti together with sister sect. Aspergillus
(formerly Eurotium) comprises osmophilic species, that are able to grow on
substrates with low water activity and in extreme environments. We
adressed the monophyly of both sections within subgenus Aspergillus and
applied a multidisciplinary approach for definition of species boundaries
in sect. Restricti. The monophyly of sections Aspergillus and Restricti
was tested on a set of 102 taxa comprising all currently accepted species
and was strongly supported by Maximum likelihood (ML) and Bayesian
inferrence (BI) analysis based on coding regions of β-tubulin (benA),
calmodulin (caM) and RNA polymerase II second largest subunit (RPB2) loci.
More than 300 strains belonging to sect. Restricti from various isolation
sources and four continents were characterized by DNA sequencing, and 193
isolates selected for phylogenetic analyses and phenotypic studies.
Species delimitation methods based on multispecies coalescent model were
employed on DNA sequences from four loci, i.e., ID region of rDNA (ITS +
LSU), caM, benA and RPB2, and supported recognition of 21 species,
including 14 new. All these species were also strongly supported in ML and
BI analyses. All recognized species can be reliably identified by all four
examined genetic loci. Phenotype analysis was performed to support the
delimitation of new species, it comprised colony characteristics on seven
cultivation media incubated at several temperatures, growth on an osmotic
gradient (six media with NaCl concentration from 0 to 25%) and analysis of
morphology including scanning electron microscopy. The micromorphology of
conidial heads, vesicle dimensions, temperature profiles and growth
parameters in osmotic gradient were useful criteria for species
identification.
beta-tubulin alignmnent of sect. Restricticalmodulin alignmnent of sect.
RestrictirDNA ID region alignmnent of sect. RestrictiRNA polymerase beta
alignmnent of sect. Restricticoncatenated alignmnent of sect.
Restricticoncatenated alignmnent of subg. Aspergilluspartitioned analysis
of sect. Restricti treepartitioned analysis of subg. Aspergillus
treeStacey (penicillioides clade)Stacey (restrictus, conicus, vitricola
clades)starBEAST