10.5061/DRYAD.3HP15
Dougherty, Matthew M.
Notre Dame University
University of Notre Dame
Larson, Eric R.
University of Illinois System
Notre Dame University
University of Notre Dame
Renshaw, Mark A.
Notre Dame University
University of Notre Dame
Gantz, Crysta A.
Notre Dame University
University of Notre Dame
Egan, Scott P.
Rice University
Erickson, Daniel M.
Notre Dame University
University of Notre Dame
Lodge, David M.
Notre Dame University
University of Notre Dame
Data from: Environmental DNA (eDNA) detects the invasive rusty crayfish
(Orconectes rusticus) at low abundances
Dryad
dataset
2017
Orconectes rusticus
quantitative PCR
exotic species
Crayfish
early warning
detection probability
early detection
occupancy estimation
Lake
non-indigenous species
2017-02-01T00:00:00Z
2017-02-01T00:00:00Z
en
https://doi.org/10.1111/1365-2664.12621
176726 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Early detection is invaluable for the cost-effective control and
eradication of invasive species, yet many traditional sampling techniques
are ineffective at the low population abundances found at the onset of the
invasion process. Environmental DNA (eDNA) is a promising and sensitive
tool for early detection of some invasive species, but its efficacy has
not yet been evaluated for many taxonomic groups and habitat types. We
evaluated the ability of eDNA to detect the invasive rusty crayfish
Orconectes rusticus, and to reflect patterns of its relative abundance, in
upper Midwest, USA inland lakes. We paired conventional baited trapping as
a measure of crayfish relative abundance with water samples for eDNA,
which were analysed in the laboratory with a qPCR assay. We modelled
detection probability for O. rusticus eDNA using relative abundance and
site characteristics as covariates, and also tested the relationship
between eDNA copy number and O. rusticus relative abundance. We detected
O. rusticus eDNA in all lakes where this species was collected by
trapping, down to low relative abundances, as well as in two lakes where
trap catch was zero. Detection probability of O. rusticus eDNA was
well-predicted by relative abundance of this species and lake water
clarity. However there was poor correspondence between eDNA copy number
and O. rusticus relative abundance estimated by trap catches. Synthesis
and applications. Our study demonstrates a field and laboratory protocol
for eDNA monitoring of crayfish invasions, with results of statistical
models that provide guidance of sampling effort and detection
probabilities for researchers in other regions and systems. We propose
eDNA be included as a tool in surveillance for invasive or imperilled
crayfishes and other benthic arthropods.
Dougherty_etal_RawDataRaw data associated with Dougherty et al. Journal of
Applied Ecology, including sample locations, baited trap catches of
crayfish, qPCR values, Sanger sequencing confirmation, and Secchi disk
depth measurements for study lakes.Dougherty_etal_Orusticus_eDNA.xlsx
United States
Wisconsin
Michigan