10.5061/DRYAD.39SC7
Ogden, Rob
Royal Zoological Society of Scotland
Gharbi, Karim
University of Edinburgh
Mugue, Nikolai
All-Russian Research Institute Fisheries and Oceanography
Martinsohn, Jann
European Commission
Senn, Helen
Royal Zoological Society of Scotland
Davey, John
University of Edinburgh
Pourkazemi, Mohammad
Sturgeon International Research Institute; PO Box 41635-3464 Rasht Iran
McEwing, Ross
Royal Zoological Society of Scotland
Eland, Cathlene
University of Edinburgh
Vidotto, Michele
University of Padua
Sergeev, Alexander
All-Russian Research Institute Fisheries and Oceanography
Congiu, Leonardo
University of Padua
Davey, J. W.
University of Edinburgh
Data from: Sturgeon conservation genomics: SNP discovery and validation
using RAD sequencing
Dryad
dataset
2013
Acipenser naccarii
DNA
Acipenser
wildlife forensics
Acipenser gueldenstaedtii
Acipenser baerii
Traceability
Acipenser persicus
caviar
2013-01-07T17:41:47Z
2013-01-07T17:41:47Z
en
https://doi.org/10.1111/mec.12234
1978236 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Caviar-producing sturgeons belonging to the genus Acipenser are considered
to be one of the most endangered species groups in the world. Continued
overfishing in spite of increasing legislation, zero catch quotas and
extensive aquaculture production have led to the collapse of wild stocks
across Europe and Asia. The evolutionary relationships among Adriatic,
Russian, Persian and Siberian sturgeons are complex because of past
introgression events and remain poorly understood. Conservation
management, traceability and enforcement suffer a lack of appropriate DNA
markers for the genetic identification of sturgeon at the species,
population and individual level. This study employed RAD sequencing to
discover and characterize single nucleotide polymorphism (SNP) DNA markers
for use in sturgeon conservation in these four tetraploid species over
three biological levels, using a single sequencing lane. Four population
meta-samples and eight individual samples from one family were barcoded
separately before sequencing. Analysis of 14.4 Gb of paired-end RAD data
focused on the identification of SNPs in the paired-end contig, with
subsequent in silico and empirical validation of candidate markers.
Thousands of putatively informative markers were identified including, for
the first time, SNPs that show population-wide differentiation between
Russian and Persian sturgeons, representing an important advance in our
ability to manage these cryptic species. The results highlight the
challenges of genotyping-by-sequencing in polyploid taxa, while
establishing the potential genetic resources for developing a new range of
caviar traceability and enforcement tools.
mec12-0761_DRYAD entry
Adriatic (Mediterranean Sea)
Azov Sea
River Lena
Caspian Sea
River Ob