10.5061/DRYAD.32152
Arnold, Markus F. F.
Massachusetts Institute of Technology
Shabab, Mohammed
Massachusetts Institute of Technology
Penterman, Jon
Massachusetts Institute of Technology
Boehme, Kevin L.
Brigham Young University
Griffitts, Joel S.
Brigham Young University
Walker, Graham C.
Massachusetts Institute of Technology
Data from: Genome-wide sensitivity analysis of the microsymbiont
Sinorhizobium meliloti to symbiotically important, defensin-like host
peptides
Dryad
dataset
2018
Symbiosis
host-microbe interactions
Antimicrobial peptides
Sinorhizobium meliloti
National Science Foundation
https://ror.org/021nxhr62
IOS-1054980
2018-06-30T00:00:00Z
2018-06-30T00:00:00Z
en
https://doi.org/10.1128/mbio.01060-17
19494788 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The model legume species Medicago truncatula expresses more than 700
nodule-specific cysteine-rich (NCR) signaling peptides that mediate the
differentiation of Sinorhizobium meliloti bacteria into nitrogen-fixing
bacteroids. NCR peptides are essential for a successful symbiosis in
legume plants of the inverted-repeat-lacking clade (IRLC) and show
similarity to mammalian defensins. In addition to signaling functions,
many NCR peptides exhibit antimicrobial activity in vitro and in vivo.
Bacterial resistance to these antimicrobial activities is likely to be
important for symbiosis. However, the mechanisms used by S. meliloti to
resist antimicrobial activity of plant peptides are poorly understood. To
address this, we applied a global genetic approach using transposon
mutagenesis followed by high-throughput sequencing (Tn-seq) to identify S.
meliloti genes and pathways that increase or decrease bacterial
competitiveness during exposure to the well-studied cationic NCR247
peptide and also to the unrelated model antimicrobial peptide polymyxin B.
We identified 78 genes and several diverse pathways whose interruption
alters S. meliloti resistance to NCR247. These genes encode the following:
(i) cell envelope polysaccharide biosynthesis and modification proteins,
(ii) inner and outer membrane proteins, (iii) peptidoglycan (PG) effector
proteins, and (iv) non-membrane-associated factors such as transcriptional
regulators and ribosome-associated factors. We describe a previously
uncharacterized yet highly conserved peptidase, which protects S. meliloti
from NCR247 and increases competitiveness during symbiosis. Additionally,
we highlight a considerable number of uncharacterized genes that provide
the basis for future studies to investigate the molecular basis of
symbiotic development as well as chronic pathogenic interactions.
Total Tn-seq read counts for all annotated S. meliloti genesTable shows
the total number of Illumina reads obtained for each S. meliloti gene for
all tested conditions. The raw results are shown as observed and then the
normalized read counts per gene are also included.all genes Tn-seq
results.xlsxAll S. meliloti genes Tn-seq results highlighting all Tn
insertionsThe table shows all Tn insertions that have been observed by
Illumina sequencing and their corresponding read counts for each
condition. The dataset includes raw read counts for each Tn insertion as
well as the normalized read counts. The dataset also includes the location
of each transposon in each corresponding gene.all genes Tn-seq results Tn
insertions.xlsx