10.5061/DRYAD.30R6P
Strese, Åke
Uppsala University
Backlund, Anders
Uppsala University
Alsmark, Cecilia
Uppsala University
Data from: A recently transferred cluster of bacterial genes in
Trichomonas vaginalis –lateral gene transfer and the fate of acquired
genes.
Dryad
dataset
2014
Trichomonas vaginalis
Lateral gene transfer (LGT)
Peptoniphilus harei
2014-06-06T14:37:47Z
2014-06-06T14:37:47Z
en
https://doi.org/10.1186/1471-2148-14-119
237505 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: Lateral Gene Transfer (LGT) has recently gained recognition as
an important contributor to some eukaryote proteomes, but the mechanisms
of acquisition and fixation in eukaryotic genomes are still uncertain. A
previously defined norm for LGTs in microbial eukaryotes states that the
majority are genes involved in metabolism, the LGTs are typically
localized one by one, surrounded by vertically inherited genes on the
chromosome, and phylogenetics shows that a broad collection of bacterial
lineages have contributed to the transferome. Results: A unique 34 kbp
long fragment with 27 clustered genes (TvLF) of prokaryote origin was
identified in the sequenced genome of the protozoan parasite Trichomonas
vaginalis. Using a PCR based approach we confirmed the presence of the
orthologous fragment in four additional T. vaginalis strains. Detailed
sequence analyses unambiguously suggest that TvLF is the result of one
single, recent LGT event. The proposed donor is a close relative to the
firmicute bacterium Peptoniphilus harei. High nucleotide sequence
similarity between T. vaginalis strains, as well as to P. harei, and the
absence of homologs in other Trichomonas species, suggests that the
transfer event took place after the radiation of the genus Trichomonas.
Some genes have undergone pseudogenization and degradation, indicating
that they may not be retained in the future. Functional annotations reveal
that genes involved in informational processes are particularly prone to
degradation. Conclusions: We conclude that, although the majority of
eukaryote LGTs are single gene occurrences, they may be acquired in
clusters of several genes that are subsequently cleansed of evolutionarily
less advantageous genes.
TvLFphylogenetic_dataFiles underlying the phylogenetic trees shown in
Additional File 2.