10.5061/DRYAD.30CN0
Epstein, Yehonatan
University of Haifa
Perry, Noam
University of Haifa
Volin, Marina
University of Haifa
Zohar-Fux, Maayan
University of Haifa
Braun, Rachel
University of Haifa
Porat-Kuperstein, Lilach
University of Haifa
Toledano, Hila
University of Haifa
Data from: miR-9a modulates maintenance and ageing of Drosophila germline
stem cells by limiting N-cadherin expression
Dryad
dataset
2017
miR-9a
miRNAome
germline
2017-09-19T16:00:00Z
2017-09-19T16:00:00Z
en
https://doi.org/10.1038/s41467-017-00485-9
10620875347 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Ageing is characterized by a decline in stem cell functionality leading to
dampened tissue regeneration. While the expression of microRNAs across
multiple species is markedly altered with age, the mechanism by which they
govern stem cell-sustained tissue regeneration is unknown. We report that
in the Drosophila testis, the conserved miR-9a is expressed in germline
stem cells and its levels are significantly elevated during ageing.
Transcriptome and functional analyses show that miR-9a directly regulates
the expression of the adhesion molecule N-cadherin (N-cad). miR-9a null
mutants maintain a higher number of stem cells even in the aged tissue.
Remarkably, this rise fails to improve tissue regeneration and results in
reduced male fertility. Similarly, overexpression of N-cad also results in
elevated stem cell number and decreased regeneration. We propose that
miR-9a downregulates N-cad to enable adequate detachment of stem cells
toward differentiation, thus providing the necessary directionality toward
terminal differentiation and spermatogenesis.
nCounter miRNA assay - w1118 1 day Replicate #1NanoString nCounter miRNA
assay from Drosophila testes, Genotype w1118, Age 1 day, Replicate
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#2NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118,
Age 1 day, Replicate #2w1118_1day_Rep2.RCCnCounter miRNA assay - w1118 15
days Replicate #1NanoString nCounter miRNA assay from Drosophila testes,
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miRNA assay - w1118 15 days Replicate #2NanoString nCounter miRNA assay
from Drosophila testes, Genotype w1118, Age 15 days, Replicate
#2w1118_15day_Rep2.RCCnCounter miRNA assay - w1118 30 days Replicate
#1NanoString nCounter miRNA assay from Drosophila testes, Genotype w1118,
Age 30 days, Replicate #1w1118_30day_Rep1.RCCnCounter miRNA assay - w1118
30 days Replicate #2NanoString nCounter miRNA assay from Drosophila
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