10.5061/DRYAD.2QG86
Meaden, Sean
University of Exeter
Paszkiewicz, Konrad
University of Exeter
Koskella, Britt
University of Exeter
Data from: The cost of phage resistance in a plant pathogenic bacterium is
context-dependent
Dryad
dataset
2015
trade-offs
Pseudomonas syringae
Evolutionary genomics
Coevolution
2015-03-23T15:08:47Z
2015-03-23T15:08:47Z
en
https://doi.org/10.1111/evo.12652
2512187325 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Parasites are ubiquitous features of living systems and many parasites
severely reduce the fecundity or longevity of their hosts. This
parasite-imposed selection on host populations should strongly favor the
evolution of host resistance, but hosts typically face a trade-off between
investment in reproductive fitness and investment in defense against
parasites. The magnitude of such a trade-off is likely to be
context-dependent, and accordingly costs that are key in shaping evolution
in nature may not be easily observable in an artificial environment. We
set out to assess the costs of phage resistance for a plant pathogenic
bacterium in its natural plant host versus in a nutrient-rich, artificial
medium. We demonstrate that mutants of Pseudomonas syringae that have
evolved resistance via a single mutational step pay a substantial cost for
this resistance when grown on their tomato plant hosts, but do not realize
any measurable growth rate costs in nutrient-rich media. This work
demonstrates that resistance to phage can significantly alter bacterial
growth within plant hosts, and therefore that phage-mediated selection in
nature is likely to be an important component of bacterial pathogenicity.
Costs of resistance CFU countsP.syringae colony forming unit counts from
homogenized tomato leaf samples. Each 'strain' relates to a
selected phage-resistant genotype or the ancestral, phage-sensitive
genotype.costs_of_resistance_cfu_data.csvOptical density growth
curvesOptical density readings of bacterial growth curves at 600nm in KB
broth.plate_reader_data.csvIllumina readsForward and reverse Illumina
reads for each bacterial line. The sensitive P.syringae DC3000 ancestral
line is called DC3ANC and all other 3 letter codes at the start of the
filename refer to each of the mutant lines generated in the
experiment.costs_geno_seqs.tar.gz