10.5061/DRYAD.2GM06
Andrei, Adrian-Stefan
Babeș-Bolyai University
Robeson, Michael S.
Oak Ridge National Laboratory
Baricz, Andreea
Babeș-Bolyai University
Coman, Cristian
Babeș-Bolyai University
Muntean, Vasile
Babeș-Bolyai University
Ionescu, Artur
Babeș-Bolyai University
Etiope, Giuseppe
Babeș-Bolyai University
Alexe, Mircea
Babeș-Bolyai University
Sicora, Cosmin Ionel
Biological Research Center, Jibou, Romania
Podar, Mircea
Oak Ridge National Laboratory
Banciu, Horia Leonard
Babeș-Bolyai University
Data from: Contrasting taxonomic stratification of microbial communities
in two hypersaline meromictic lakes
Dryad
dataset
2015
Archaea
hypersaline meromictic lakes
biogeochemical cycling
methanogenesis
prokaryotic diversity
ultrasmall uncultivated Archaea
deep coverage SSU rDNA amplicon sequencing
2015-12-29T00:00:00Z
2015-12-29T00:00:00Z
en
https://doi.org/10.1038/ismej.2015.60
1267500 bytes
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Hypersaline meromictic lakes are extreme environments in which water
stratification is associated with powerful physicochemical gradients and
high salt concentrations. Furthermore, their physical stability coupled
with vertical water column partitioning makes them important research
model systems in microbial niche differentiation and biogeochemical
cycling. Here, we compare the prokaryotic assemblages from Ursu and Fara
Fund hypersaline meromictic lakes (Transylvanian Basin, Romania) in
relation to their limnological factors and infer their role in elemental
cycling by matching taxa to known taxon-specific biogeochemical functions.
To assess the composition and structure of prokaryotic communities and the
environmental factors that structure them, deep-coverage small subunit
(SSU) ribosomal RNA (rDNA) amplicon sequencing, community domain-specific
quantitative PCR and physicochemical analyses were performed on samples
collected along depth profiles. The analyses showed that the lakes
harbored multiple and diverse prokaryotic communities whose distribution
mirrored the water stratification patterns. Ursu Lake was found to be
dominated by Bacteria and to have a greater prokaryotic diversity than
Fara Fund Lake that harbored an increased cell density and was populated
mostly by Archaea within oxic strata. In spite of their contrasting
diversity, the microbial populations indigenous to each lake pointed to
similar physiological functions within carbon degradation and sulfate
reduction. Furthermore, the taxonomy results coupled with methane
detection and its stable C isotope composition indicated the presence of a
yet-undescribed methanogenic group in the lakes’ hypersaline
monimolimnion. In addition, ultrasmall uncultivated archaeal lineages were
detected in the chemocline of Fara Fund Lake, where the recently proposed
Nanohaloarchaeota phylum was found to thrive.
Information for Andrei et al. articleInformation related to the article:
"Contrasting taxonomic stratification of microbial communities in two
hypersaline meromictic lakes" by Andrei et al.DataDryad.pdf
Romania
Transylvanian Basin