10.5061/DRYAD.2F7V9
Puncher, Gregory Neils
University of Bologna
University of New Brunswick
Ghent University
Cariani, Alessia
University of Bologna
Maes, Gregory E.
KU Leuven
James Cook University
Van Houdt, Jeroen
KU Leuven
Herten, Koen
KU Leuven
Cannas, Rita
University of Cagliari
Rodriguez-Ezpeleta, Naiara
Marine Research Division; AZTI Tecnalia; Pasaia Gipuzkoa Spain
Albaina, Aitor
University of the Basque Country
Estonba, M. Andone
University of the Basque Country
Lutcavage, Molly
University of Massachusetts Amherst
Hanke, Alex
Fisheries and Oceans Canada
Rooker, Jay
Texas A&M University
Texas A&M University at Galveston
Franks, James S.
University of Southern Mississippi
Quattro, Joseph M.
University of South Carolina
Basilone, Gualtiero
National Research Council
Fraile, Igaratza
University of Genoa
Laconcha, Urtzi
University of the Basque Country
Goñi, Nicolas
University of Bologna
Kimoto, Ai
University of Cagliari
Macías, A. David
Spanish Institute of Oceanography
Alemany, Francisco
Spanish Institute of Oceanography
Deguara, Simeon
Istanbul University
Zgozi, Salem W.
Marine Biology Research Center; Tripoli-Tajura Libya
Garibaldi, Fulvio
University of Genoa
Oray, Isik K.
Istanbul University
Karakulak, F. Saadet
Istanbul University
Abid, Noureddine
National Institute of Fisheries Research; Regional Centre of Tangier;
Tanger Morocco
Santos, Miguel N.
Portuguese Sea and Atmosphere Institute
Addis, Piero
University of Cagliari
Arrizabalaga, Haritz
Marine Research Division; AZTI Tecnalia; Pasaia Gipuzkoa Spain
Tinti, Fausto
University of Bologna
Data from: Spatial dynamics and mixing of bluefin tuna in the Atlantic
Ocean and Mediterranean Sea revealed using next generation sequencing
Dryad
dataset
2018
Genomics/Proteomics
Thunnus thynnus
2018-01-31T19:17:58Z
2018-01-31T19:17:58Z
en
https://doi.org/10.1111/1755-0998.12764
919892 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The Atlantic bluefin tuna is a highly migratory species emblematic of the
challenges associated with shared fisheries management. In an effort to
resolve the species’ stock dynamics, a genome-wide search for spatially
informative single nucleotide polymorphisms (SNPs) was undertaken, by way
of sequencing reduced representation libraries. An allele frequency
approach to SNP discovery was used, combining the data of 555 larvae and
young-of-the-year (LYOY) into pools representing major geographical areas
and mapping against a newly assembled genomic reference. From a set of
184,895 candidate loci, 384 were selected for validation using 167 LYOY. A
highly discriminatory genotyping panel of 95 SNPs was ultimately developed
by selecting loci with the most pronounced differences between western
Atlantic and Mediterranean Sea LYOY. The panel was evaluated by genotyping
a different set of LYOY (n= 326) and from these 77.8% and 82.1% were
correctly assigned to western Atlantic and Mediterranean Sea origins,
respectively. The panel revealed temporally persistent differentiation
among LYOY from the western Atlantic and Mediterranean Sea (FST = 0.008,
p=0.034). The composition of six mixed feeding aggregations in the
Atlantic Ocean and Mediterranean Sea was characterized using genotypes
from medium (n = 184) and large (n = 48) adults, applying population
assignment and mixture analyses. The results provide evidence of
persistent population structuring across broad geographic areas and
extensive mixing in the Atlantic Ocean, particularly in the mid-Atlantic
Bight and Gulf of St. Lawrence. The genomic reference and genotyping tools
presented here constitute novel resources useful for future research and
conservation efforts.
Puncher et al_Thunnus thynnus_95loci_723individuals_27samplesPuncher et
al_Thunnus thynnus_221loci_167individuals_9samplesPuncher et al_Thunnus
thynnus_384 SNPs with flanking sequences"Puncher et al_Thunnus
thynnus_384 SNPs with flanking sequences.txt" contains the flanking
sequences of 384 SNPs discovered using reduced representation sequence
libraries, 221 of which passed quality criteria and 95 that were used for
population structure analysis.
Atlantic Ocean
Gulf of Mexico
Mediterranean Sea