10.5061/DRYAD.2752G
Yoshido, Atsuo
Institute of Entomology
Marec, Frantisek
Sahara, Ken
Iwate University
Data from: The fate of W chromosomes in hybrids between wild silkmoths,
Samia cynthia ssp.: no role in sex determination and reproduction
Dryad
dataset
2015
2015-11-25T19:23:21Z
2015-11-25T19:23:21Z
en
https://doi.org/10.1038/hdy.2015.110
1850 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Moths and butterflies (Lepidoptera) have sex chromosome systems with
female heterogamety (WZ/ZZ or derived variants). The maternally inherited
W chromosome is known to determine female sex in the silkworm, Bombyx
mori. However, little is known about the role of W chromosome in other
lepidopteran species. Here we describe two forms of the W chromosome, W
and neo-W, that are transmitted to both sexes in offspring of hybrids from
reciprocal crosses between subspecies of wild silkmoths, Samia cynthia. We
performed crosses between S. c. pryeri (2n=28, WZ/ZZ) and S. c. walkeri
(2n=26, neo-Wneo-Z/neo-Zneo-Z) and examined fitness and sex chromosome
constitution in their hybrids. The F1 hybrids of both reciprocal crosses
had reduced fertility. Fluorescence in situ hybridization revealed not
only the expected sex chromosome constitutions in the backcross and F2
hybrids of both sexes but also females without the W (or neo-W) chromosome
and males carrying the W (or neo-W) chromosome. Furthermore, crosses
between the F2 hybrids revealed no association between the presence or
absence of W (or neo-W) chromosome and variations in the hatchability of
their eggs. Our results clearly suggest that the W (or neo-W) chromosome
of S. cynthia ssp. plays no role in sex determination and reproduction,
and thus does not contribute to the formation of reproductive barriers
between different subspecies.
Hatchability of eggs in crosses between Samia cynthia ssp.These are
original dataset to Figure 2 and Supplementary Table S1. Hatchability of
eggs in the control (C), parental (P) and F1 crosses, and backcrosses (B1)
between Samia cynthia walkeri (SCW) and Samia cynthia pryeri
(SCP).original dataset to Figure 2 and supplementary Table S1.csv