10.5061/DRYAD.2182N
Beamish, Christine A.
Western University
Children’s Health Research Institute
Lawson Health Research Institute
Zhang, Linhao
Lawson Health Research Institute
Sichuan University
Szlapinski, Sandra K.
Western University
Lawson Health Research Institute
Strutt, Brenda J.
Children’s Health Research Institute
Hill, David J.
Western University
Children’s Health Research Institute
Lawson Health Research Institute
Data from: An increase in immature β-cells lacking Glut2 precedes the
expansion of β-cell mass in the pregnant mouse
Dryad
dataset
2018
proliferation
Glut2
Pancreas
Pregnancy
β-cell
islet
progenitor cell
2018-07-13T00:00:00Z
2018-07-13T00:00:00Z
en
https://doi.org/10.1371/journal.pone.0182256
2490089 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
A compensatory increase in β-cell mass occurs during pregnancy to counter
the associated insulin resistance, and a failure in adaptation is thought
to contribute to gestational diabetes. Insulin-expressing but
glucose-transporter-2-low (Ins+Glut2LO) progenitor cells are present in
mouse and human pancreas, being predominantly located in extra-islet
β-cell clusters, and contribute to the regeneration of the endocrine
pancreas following induced ablation. We therefore sought to investigate
the contribution of Ins+Glut2LO cells to β-cell mass expansion during
pregnancy. Female C57Bl/6 mice were time mated and pancreata were
collected at gestational days (GD) 6, 9, 12, 15, and 18, and postpartum D7
(n = 4/time-point) and compared to control (non-pregnant) animals. Beta
cell mass, location, proliferation (Ki67+), and proportion of Ins+Glut2LO
cells were measured using immunohistochemistry and bright field or
confocal microscopy. Beta cell mass tripled by GD18 and β-cell
proliferation peaked at GD12 in islets (≥6 β-cells) and small β-cell
clusters (1–5 β-cells). The proportion and fraction of Ins+Glut2LO cells
undergoing proliferation increased significantly at GD9 in both islets and
clusters, preceding the increase in β-cell mass and proliferation, and
their proliferation within clusters persisted until GD15. The overall
number of clusters increased significantly at GD9. Quantitative PCR showed
a significant increase in Pdx1 presence at GD9 vs. GD18 or control
pancreas, and Pdx1 was visualized by immunohistochemistry within both
Ins+Glut2LO and Ins+Glut2HI cells within clusters. These results indicate
that Ins+Glut2LO cells are likely to contribute to β-cell mass expansion
during pregnancy.
GTT dataPrism format fileGTT.pzfMafB expression dataPrism format
fileMafB.pzfNkx6.1 expressionPrism format
fileNkx6.1.pzfPdx1expressionPrism format filePdx1.pzfPdx1 protein levels
ELISAPrism format filePdx1 ELISA minimal dat set.pzfSummary cell histology
dataMicrosoft Excel fileSummary data PLos 1 subm.xlsx