10.5061/DRYAD.215JD
Sheppard, Samuel K.
University of Oxford
Swansea University
Didelot, Xavier
Imperial College London
Jolley, Keith A.
University of Oxford
Darling, Aaron E.
University of Oxford
Kelly, David J.
University of Sheffield
Cody, Alison
University of Oxford
Colles, Frances M.
University of Oxford
Strachan, Norval J.C.
University of Aberdeen
Ogden, Iain D.
University of Aberdeen
Forbes, Ken
University of Aberdeen
French, Nigel P.
Massey University
Carter, Philip
Statens Serum Institut
Miller, William G.
United States Department of Agriculture
McCarthy, Noel D.
University of Oxford
Owen, Robert
Health Protection Agency's Centre for Infections 61 Colindale
Avenue London NW9 5EQ UK
Litrup, Eva
Statens Serum Institut
Egholm, Michael
Pall Corporation 25 Harbor Park Drive Port Washington NY 11050 USA
Bentley, Stephen D.
Wellcome Trust
Parkhill, Julian
Wellcome Trust
Maiden, Martin C. J.
University of Oxford
Falush, Daniel
29 Glebe Road London SW130DZ UK
Affourtit, Jason P.
Ion Torrent 246 Goose Lane Guilford CT 06437 USA
Strachan, Norval J. C.
University of Aberdeen
Pascoe, Ben
Swansea University
Meric, Guillaume
Swansea University
Data from: Progressive genome-wide introgression in agricultural
Campylobacter coli
Dryad
dataset
2012
whole genome sequencing
2012-11-09T15:29:38Z
2012-11-09T15:29:38Z
en
https://doi.org/10.1111/mec.12162
18542480 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Hybridization between distantly related organisms can facilitate rapid
adaptation to novel environments, but is potentially constrained by
epistatic fitness interactions among cell components. The zoonotic
pathogens Campylobacter coli and C. jejuni differ from each other by
around 15% at the nucleotide level, corresponding to an average of nearly
40 amino acids per protein-coding gene. Using whole genome sequencing, we
show that a single C. coli lineage, which has successfully colonized an
agricultural niche, has been progressively accumulating C. jejuni DNA.
Members of this lineage belong to two groups, the ST-828 and ST-1150
clonal complexes. The ST-1150 complex is less frequently isolated and has
undergone a substantially greater amount of introgression leading to
replacement of up to 23% of the C. coli core genome as well as import of
novel DNA. By contrast, the more commonly isolated ST-828 complex bacteria
have 10–11% introgressed DNA, and C. jejuni and nonagricultural C. coli
lineages each have <2%. Thus, the C. coli that colonize
agriculture, and consequently cause most human disease, have hybrid
origin, but this cross-species exchange has so far not had a substantial
impact on the gene pools of either C. jejuni or nonagricultural C. coli.
These findings also indicate remarkable interchangeability of basic
cellular machinery after a prolonged period of independent evolution.
BIGS_sequencesWhole genome sequencing reads for BIGSdb isolates 1-30
(isolates 26,27,28,29 are already published with accession numbers:
NC_009839; NC_008787; NC_003912; NC_002163). For isolates 1-3 de novo 454
sequencing was assembled using Newbler (April 2009). For isolates 4-25 and
30 de novo illumnia sequencing was assembled using Velvet 1.0.