10.5061/DRYAD.1QH4C
Findlay, Amy S.
University of Aberdeen
Panzica, D. Alessio
University of Aberdeen
Walczysko, Petr
University of Aberdeen
Holt, Amy B.
University of Aberdeen
Henderson, Deborah J.
Newcastle University
West, John D.
University of Edinburgh
Rajnicek, Ann M.
University of Aberdeen
Collinson, J. Martin
University of Aberdeen
Data from: The core planar cell polarity gene, Vangl2, directs adult
corneal epithelial cell alignment and migration
Dryad
dataset
2016
Vangl2
Cornea
Planar cell polarity
Epithelium
2016-10-17T16:13:00Z
2016-10-17T16:13:00Z
en
https://doi.org/10.1098/rsos.160658
15940971 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
This study shows that the core planar cell polarity (PCP) genes direct the
aligned cell migration in the adult corneal epithelium, a stratified
squamous epithelium on the outer surface of the vertebrate eye. Expression
of multiple core PCP genes was demonstrated in the adult corneal
epithelium. PCP components were manipulated genetically and
pharmacologically in human and mouse corneal epithelial cells in vivo and
in vitro. Knockdown of VANGL2 reduced the directional component of
migration of human corneal epithelial (HCE) cells without affecting speed.
It was shown that signalling through PCP mediators, dishevelled,
dishevelled-associated activator of morphogenesis and Rho-associated
protein kinase directs the alignment of HCE cells by affecting
cytoskeletal reorganization. Cells in which VANGL2 was disrupted tended to
misalign on grooved surfaces and migrate across, rather than parallel to
the grooves. Adult corneal epithelial cells in which Vangl2 had been
conditionally deleted showed a reduced rate of wound-healing migration.
Conditional deletion of Vangl2 in the mouse corneal epithelium ablated the
normal highly stereotyped patterns of centripetal cell migration in vivo
from the periphery (limbus) to the centre of the cornea. Corneal opacity
owing to chronic wounding is a major cause of degenerative blindness
across the world, and this study shows that Vangl2 activity is required
for directional corneal epithelial migration.
Figure 2Prism files for Figure 2 - see read me.Figure S3Prism Files for
Fig. S3 - see read me.Figure S4Prism Files for Figure S4 - see read
meFigure 3AFigure 3BFigure 3CFigure 3DFigure 4AFigure 4BFigure 4CFigure
4DFigure 4EFigure 4FPRISM FILES