10.5061/DRYAD.1P7SF
Kijas, James W.
Commonwealth Scientific and Industrial Research Organisation
Hadfield, Tracy
Utah State University
Naval Sanchez, Marina
Commonwealth Scientific and Industrial Research Organisation
Cockett, Noelle
Utah State University
Data from: Genome-wide association reveals the locus responsible for
four-horned ruminant
Dryad
dataset
2016
horn morphology
Ovis aries
Sheep
2016-11-30T00:00:00Z
2016-11-30T00:00:00Z
en
https://doi.org/10.1111/age.12409
12129769 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Phenotypic variability in horn characteristics, such as their size, number
and shape, offers the opportunity to elucidate the molecular basis of horn
development. The objective of this study was to map the genetic
determinant controlling the production of four horns in two breeds, Jacob
sheep and Navajo-Churro, and examine whether an eyelid abnormality
occurring in the same populations is related. Genome-wide association
mapping was performed using 125 animals from the two breeds that contain
two- and four-horned individuals. A case–control design analysis of 570
712 SNPs genotyped with the ovine HD SNP Beadchip revealed a strong
association signal on sheep chromosome 2. The 10 most strongly associated
SNPs were all located in a region spanning Mb positions 131.9–132.6,
indicating the genetic architecture underpinning the production of four
horns is likely to involve a single gene. The closest genes to the most
strongly associated marker (OAR2_132568092) were MTX2 and the HOXD
cluster, located approximately 93 Kb and 251 Kb upstream respectively. The
occurrence of an eyelid malformation across both breeds was restricted to
polled animals and those carrying more than two horns. This suggests the
eyelid abnormality may be associated with departures from the normal
developmental production of two-horned animals and that the two conditions
are developmentally linked. This study demonstrated the presence of
separate loci responsible for the polled and four-horned phenotypes in
sheep and advanced our understanding of the complexity that underpins horn
morphology in ruminants.
GWAS_SNP_GenotypesSNP genotypes formatted as ped and map files for
analysis using PLINK v1.7 or later.Dryad_Submission.7z