10.5061/DRYAD.1NC50
Nadeau, Nicola
University of Cambridge
Ruiz, Mayte
3Department of Biology and Center for Applied Tropical Ecology and
Conservation, University of Puerto Rico, Rio Piedras, San Juan, Puerto
Rico 00921;
Salazar, Patricio
University of Cambridge
Counterman, Brian
5Department of Biology, Mississippi State University, Mississippi 39762, USA;
Medina, Jose Alejandro
6High Performance Computing Facility, University of Puerto Rico, San
Juan, Puerto Rico, 00921;
Ortiz-Zuazaga, Humberto
6High Performance Computing Facility, University of Puerto Rico, San
Juan, Puerto Rico, 00921;
Morrison, Anna
University of Cambridge
McMillan, W. Owen
8Smithsonian Tropical Research Institute, Apartado 0843-03092, Balboa,
Ancón, Panama
Jiggins, Chris D.
University of Cambridge
Papa, Riccardo
3Department of Biology and Center for Applied Tropical Ecology and
Conservation, University of Puerto Rico, Rio Piedras, San Juan, Puerto
Rico 00921;
Data from: Population genomics of parallel hybrid zones in the mimetic
butterflies, H. melpomene and H. erato
Dryad
dataset
2015
Heliconius timareta
genome-wide association mapping
Convergent evolution
Heliconius erato
Heliconius melpomene
2015-05-02T00:00:00Z
2015-05-02T00:00:00Z
en
https://doi.org/10.1101/gr.169292.113
1365752750 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Hybrid zones can be valuable tools for studying evolution and identifying
genomic regions responsible for adaptive divergence and underlying
phenotypic variation. Hybrid zones between subspecies of Heliconius
butterflies can be very narrow and are maintained by strong selection
acting on colour pattern. The co-mimetic species H. erato and H. melpomene
have parallel hybrid zones where both species undergo a change from one
colour pattern form to another. We use restriction associated DNA
sequencing to obtain several thousand genome wide sequence markers and use
these to analyse patterns of population divergence across two pairs of
parallel hybrid zones in Peru and Ecuador. We compare two approaches for
analysis of this type of data; alignment to a reference genome and de novo
assembly, and find that alignment gives the best results for species both
closely (H. melpomene) and distantly (H. erato, ~15% divergent) related to
the reference sequence. Our results confirm that the colour pattern
controlling loci account for the majority of divergent regions across the
genome, but we also detect other divergent regions apparently unlinked to
colour pattern differences. We also use association mapping to identify
previously unmapped colour pattern loci, in particular the Ro locus.
Finally, we identify a new cryptic population of H. timareta in Ecuador,
which occurs at relatively low altitude and is mimetic with H. melpomene
malleti.
Heliconius erato emma (Peru) imagesPeru_Hera_emma.tifHeliconius erato
favorinus (Peru) imagesPeru_Hera_favorinus.tifHeliconius erato hybrids
(Peru) imagesPeru_Hera_hybrids.tifHeliconius melpomene aglaope (Peru)
imagesPeru_Hmel_aglaope.tifHeliconius melpomene amaryllis (Peru)
imagesPeru_Hmel_amaryllis.tifHeliconius melpomene hybrids (Peru)
imagesPeru_Hmel_hybrids.tifNadeau_GR_2014_scriptsZipped archive of scripts
Ecuador
Peru