10.5061/DRYAD.18GT9
Cuco, Ana Patrícia
University of Aveiro
Abrantes, Nelson
University of Aveiro
Gonçalves, Fernando
University of Aveiro
Wolinska, Justyna
Freie Universität Berlin
Leibniz Institute of Freshwater Ecology and Inland Fisheries
Castro, Bruno B.
University of Aveiro
University of Minho
Data from: Interplay between fungicides and parasites: tebuconazole, but
not copper, suppresses infection in a Daphnia-Metschnikowia experimental
model
Dryad
dataset
2018
environmental contamination
copper sulphate
Fungicides
Anti-fungal agents
multiple stressors
tebuconazole
Daphnia galeata
Daphnia galeata x longispina
host-parasite relationship
pollution-disease interaction
2018-02-10T00:00:00Z
2018-02-10T00:00:00Z
en
https://doi.org/10.1371/journal.pone.0172589
76217 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Natural populations are commonly exposed to complex stress scenarios,
including anthropogenic contamination and their biological enemies (e.g.,
parasites). The study of the pollutant-parasite interplay is especially
important, given the need for adequate regulations to promote improved
ecosystem protection. In this study, a host-parasite model system (Daphnia
spp. and the microparasitic yeast Metschnikowia bicuspidata) was used to
explore the reciprocal effects of contamination by common agrochemical
fungicides (copper sulphate and tebuconazole) and parasite challenge. We
conducted 21-day life history experiments with two host clones exposed to
copper (0.00, 25.0, 28.8 and 33.1 μg L-1) or tebuconazole (0.00, 154, 192
and 240 μg L-1), in the absence or presence of the parasite. For each
contaminant, the experimental design consisted of 2 Daphnia clones × 4
contaminant concentrations × 2 parasite treatments × 20 replicates = 320
experimental units. Copper and tebuconazole decreased Daphnia survival or
reproduction, respectively, whilst the parasite strongly reduced host
survival. Most importantly, while copper and parasite effects were mostly
independent, tebuconazole suppressed infection. In a follow-up experiment,
we tested the effect of a lower range of tebuconazole concentrations
(0.00, 6.25, 12.5, 25.0, 50.0 and 100 μg L-1) crossed with increasing
parasite challenge (2 Daphnia clones × 6 contaminant concentrations × 2
parasite levels × 20 replicates = 480 experimental units). Suppression of
infection was confirmed at environmentally relevant concentrations
(> 6.25 μg L-1), irrespective of the numbers of parasite challenge.
The ecological consequences of such a suppression of infection include
interferences in host population dynamics and diversity, as well as
community structure and energy flow across the food web, which could
upscale to ecosystem level given the important role of parasites.
Experimental data for Cuco et al.Excel file with data from two
experimental steps: first, we tested the effect of each contaminant
(copper sulphate or tebuconazole) in the presence or absence of the
microparasitic yeast (Metschnikowia bicuspidata) in two host clones
(Daphnia spp., clones 12 and 47); second, we tested the effect of
increasing the number or parasite challenge (from one to two) on the
response of the host-parasite system to tebuconazole (follow-up
experiment). Data represent life history records of individual Daphnia
(except for per capita rate of increase, where jack-knife pseudo-values
are shown).data file for Cuco et al.xlsx