10.5061/DRYAD.155DF
Yamamoto, Kazuki
Hokkaido University
Oda, Yuji
Hokkaido University
Haseda, Akane
Hokkaido University
Fujito, Satoshi
Hokkaido University
Mikami, Tetsuo
Hokkaido University
Onodera, Yasuyuki
Hokkaido University
Data from: Molecular evidence that the genes for dioecism and monoecism in
Spinacia oleracea L. are located at different loci in a chromosomal region
Dryad
dataset
2013
monoecy
sex chromosome
Dioecy; Monoecy; Spinach; Spinacia oleracea; Sex chromosome
Spinacia oleracea
Spinach
2013-09-30T14:15:57Z
2013-09-30T14:15:57Z
en
https://doi.org/10.1038/hdy.2013.112
45862 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Spinach (Spinacia oleracea L.) is widely known to be dioecious. However,
monoecious plants can also occur in this species. Sex expression in
dioecious spinach plants is controlled by a single gene pair termed X and
Y. Our previous study showed that a single, incompletely dominant gene,
which controls the monoecious condition in spinach line 03–336, should be
allelic or linked to X/Y. Here, we developed 19 AFLP markers closely
linked to the monoecious gene. The AFLP markers were mapped to a 38.2-cM
chromosomal region that included the monoecious gene, which is bracketed
between flanking markers with a distance of 7.1 cM. The four AFLP markers
developed in our studies were converted into sequence-characterized
amplified region (SCAR) markers, which are linked to both the monoecious
gene and Y and are common to both populations segregating for the genes.
Linkage analysis using the SCAR markers suggested that the monoecious gene
(M) and Y are located in different intervals, between different marker
pairs. Analysis of populations segregating for both M and Y also directly
demonstrates linkage of the genes at a distance of ~12 cM. The data
presented in this study may be useful for breeding dioecious and highly
male monoecious lines utilized as the pollen parents for hybrid seed
production, as well as for studies of the evolutionary history of sexual
systems in this species, and can provide a molecular basis for positional
cloning of the sex-determining genes.
Haseda et al._Mapping Data