10.5061/DRYAD.110NT
Marden, James H.
Pennsylvania State University
Fescemyer, Howard W.
Pennsylvania State University
Schilder, Rudolf J.
Pennsylvania State University
Doerfler, William R.
Pennsylvania State University
Vera, Juan Cristobal
Wheat, Christopher W.
University of Helsinki
Data from: Genetic variation in HIF signaling underlies quantitative
variation in physiological and life history traits within lowland
butterfly populations
Dryad
dataset
2012
Melitaea cinxia
polymorphism
2012-10-26T18:36:23Z
2012-10-26T18:36:23Z
en
https://doi.org/10.1111/evo.12004
150016 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Oxygen conductance to the tissues determines aerobic metabolic performance
in most eukaryotes but has cost/benefit tradeoffs. Here we examine in
lowland populations of a butterfly a genetic polymorphism affecting oxygen
conductance via the hypoxia inducible factor (HIF) pathway, which senses
intracellular oxygen and controls the development of oxygen delivery
networks. Genetically distinct clades of Glanville fritillary (Melitaea
cinxia) across a continental scale maintain, at intermediate frequencies,
alleles in a metabolic enzyme (succinate dehydrogenase, SDH) that
regulates hypoxia inducible factor (HIF-1α). One Sdhd allele was
associated with reduced SDH activity rate, two-fold greater
cross-sectional area of tracheoles in flight muscle, and better flight
performance. Butterflies with less tracheal development had greater
post-flight hypoxia signaling, swollen, disrupted mitochondria and
accelerated aging of flight metabolic performance. Allelic associations
with metabolic and aging phenotypes were replicated in samples from
different clades. Experimentally elevated succinate in pupae increased the
abundance of hypoxia inducible factor (HIF-1α) and expression of genes
responsive to HIF activation, including tracheal morphogenesis genes.
These results indicate that the hypoxia inducible pathway, even in lowland
populations, can be an important axis for genetic variation underlying
intraspecific differences in oxygen delivery, physiological performance
and life history.
enzyme rate and SDHD transcript qPCRMitochondrial traitsSpain flight
metabolismFrench males flight metabolism etcNrv2 and Phd expression in new
adultsPhd_Noc_Nrv2_Hif1a in pupae
Finland
France
Spain