10.5061/DRYAD.0PV6570
Liu, Chang
University of Georgia
Gibson, Amanda Kyle
Emory University
Timper, Patricia
United States Department of Agriculture
Morran, Levi T.
Emory University
Tubbs, R. Scott
University of Georgia
Data from: Rapid change in host specificity in a field population of the
biological control organism Pasteuria penetrans
Dryad
dataset
2018
Pasteuria
Meloidogyne arenaria
root-knot nematode
crop rotation
Meloidogyne
Pasteuria penetrans
Peanut
Coevolution
2018-12-04T16:50:40Z
2018-12-04T16:50:40Z
en
https://doi.org/10.1111/eva.12750
51823 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
In biological control, populations of both the biological control agent
and the pest have the potential to evolve, and even to coevolve. This
feature marks the most powerful and unpredictable aspect of biological
control strategies. In particular, evolutionary change in host specificity
of the biological control agent could increase or decrease its efficacy.
Here, we tested for change in host specificity in a field population of
the biological control organism Pasteuria penetrans. Pasteuria penetrans
is an obligate parasite of the plant parasitic nematodes Meloidogyne spp.,
which are major agricultural pests. From 2013 through 2016, we collected
yearly samples of P. penetrans from eight plots in a field infested with
M. arenaria. Plots were planted either with peanut (Arachis hypogaea) or
with a rotation of peanut and soybean (Glycine max). To detect temporal
change in host specificity, we tested P. penetrans samples annually for
their ability to attach to (and thereby infect) four clonal lines of M.
arenaria. After controlling for temporal variation in parasite abundance,
we found that P. penetrans from each of the eight plots showed temporal
variation in their attachment specificity to the clonal host lines. The
trajectories of change in host specificity were largely unique to each
plot. This result suggests that local forces, at the level of individual
plots, drive change in specificity. We hypothesize that coevolution with
local M. arenaria hosts may be one such force. Lastly, we observed an
overall reduction in attachment using samples from rotation relative to
peanut plots. This result may reflect lower abundance of P. penetrans
under crop rotation, potentially due to suppressed density of host
nematodes. As a whole, the results show local change in specificity on a
yearly basis, consistent with evolution of a biological control organism
in its ability to infect and suppress its target pest.
Data_for_ChangGibson2018_EvolAppData file for Chang, Gibson et al.
Evolutionary Applications. Each row presents the results of the bioassay
of 25 nematodes in each clonal line x plot x treatment x year combination.
Column "Year" indicates the year the soil sample was collected.
Column "SEM" (single-egg mass line) indicates the clonal host
line used for testing the soil (or the heterogeneous Greenhouse line).
Column "Plot" indicates which plot the soil sample was drawn
from. Column "Treatment" indicates whether or not the plot was
part of the Peanut or Rotation treatments. Column "Replicate" is
a repeat of the "Plot" column, but with unique identifiers.
Columns "A" through "AD" show the number of endospores
attached to individual nematodes within each of the combinations indicated
by the beginning columns.ChangGibsonetal2018_RScriptsR Markdown file
containing scripts for analyses and figures in the paper.
USA
Georgia
Tifton