10.5061/DRYAD.0KH1F8J
Alsuwayian, Nahlah
University of Western Australia
Mohring, Margaret
University of Western Australia
Cambridge, Marion
University of Western Australia
Coleman, Melinda
Southern Cross University
Kendrick, Gary
University of Western Australia
Wernberg, Thomas
University of Western Australia
Data from: A review of protocols for the experimental release of kelp
(Laminariales) zoospores
Dryad
dataset
2020
Laminariales
kelp
current
Holocene
2020-05-29T00:00:00Z
en
https://doi.org/10.1002/ece3.5389
273299 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Kelps (order Laminariales) are foundation species in temperate and arctic
seas globally, but they are in decline in many places. Laminarian kelp
have an alternation of generations and this poses challenges for
experimental studies due to the difficulties in achieving zoospore release
and gametophyte growth. Here we review and synthesize the protocols that
have been used to induce zoospore release in kelps to identify
commonalities and provide guidance on best practices. We found 171 papers,
where zoospore release was induced in four kelp families from 35 different
ecoregions. The most commonly treated family was Laminariaceae, followed
by Lessoniaceae and the most studied ecoregion was Central Chile, followed
by the Southern California Bight. Zoospore release generally involved
three steps: a pre-treatment which included cleaning of the reproductive
tissue to eliminate epiphytic organisms, followed by desiccation of the
tissue, and finally a post-desiccation immersion of the reproductive
material in a seawater medium for zoospore release. Despite these
commonalities, there was a high degree of variation in the detail within
each of these steps, even among studies within genera and from the same
ecoregions. This suggests that zoospore release may be relatively
insensitive across the Laminariales. Nevertheless, greater attention to
standardisation of protocols would facilitate comparisons of kelp zoospore
release across species and locations. Based on practicality and most
common practices, we suggest zoospore release should be achieved through
1) wiping clean the reproductive material, 2) desiccating the reproductive
material by wrapping in moist paper towels for 12 hours at room
temperature in darkness, and 3) submerging the tissue in seawater medium
for one hour at room temperature in darkness. The choice of seawater
medium should depend on the actual experiment conducted. Standardized
protocols will facilitate broad scale comparative studies across this key
taxa.
Comprehensive list of the journal articles examining the microscopic
stages on LaminarialesThe data table available through this link contains
a comprehensive list of the journal articles examining the microscopic
stages on Laminariales, displaying the full description of the species
studied; geographic location (GPS position) and ecoregion; collection
date; sea surface temperature; details concerning the protocol used to
induce zoospore release; aim of the research; and the reference.Alsuwaiyan
et al spore release Dryad raw data table.docx
Global