10.5061/DRYAD.0735S
Horn, Rebekah L.
Trent University
Cowley, David E.
New Mexico State University
Data from: Self-fertilization and the role of males in populations of
tadpole shrimp (Branchiopoda: Notostraca: Triops)
Dryad
dataset
2016
progeny array
Parthenogenesis
Triops newberryi
Branchiopoda
androdioecy
hermaphrodite
simulations
2016-06-27T14:51:04Z
2016-06-27T14:51:04Z
en
https://doi.org/10.1093/jhered/esw040
78964 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Self-fertilization has both negative and positive fitness effects on
species evolution. Selfing can increase inbreeding depression, thereby
decreasing genetic diversity. In contrast, self-fertilization can preserve
beneficial gene combinations and facilitate colonization success. Within
the class of crustaceans Branchiopoda, selfing is a primary reproductive
mode. Some species of Triops, in the family Notostraca, are a few of the
animal species thought to have a mixed mating system between
hermaphrodites and males termed androdioecy. The objective of this study
is to validate the reproductive mode utilized by Triops newberryi in
southern New Mexico by the use of progeny arrays and population
simulations. Individuals were reared in the lab from dried soil collected
from temporary ponds inhabited by T. newberryi. The adults reared and the
encysted embryos contained within their brood pouches were genotyped using
seven T. newberryi specific microsatellite markers to determine the
relatedness between parent and offspring. Overall microsatellite diversity
was low with few heterozygous individuals and limited polymorphisms.
Simulated populations and allele segregation analysis suggest
hermaphroditism is the primary reproductive mode for T. newberryi. In
addition, based on the offspring’s alleles, there was no direct evidence
that a male (ovisacless) T. newberryi outcrossed with a female. Population
simulations further suggest that the rate of successful outcrossing events
must be low and could explain why outcrossing was not observed in the
laboratory rearing trials.
Horn&Cowley_Tnewberryi GenotypesThe microsatellite genotypes for
the adult and offspring samples from the Horn and Cowley
manuscript.Horn&Cowley_Easypop_RunWindowsA text file containing
the input parameters for the program EASYPOP used to generate the
simulated datasets.Horn&Cowley_GeneticStatistics_SimulationsA file
containing the genetic statistics (FIS, number of alleles, levels of
heterozygosity, etc.) generated from the simulated datasets. This data was
used to build figure 1 in the manuscript associated with this entry, i.e.
Horn and Cowley(2016).
Southern New Mexico
United States of America