10.5061/DRYAD.05QFTTF1W
Zindel, Joel
0000-0003-3685-9338
University of Bern
Peiseler, Moritz
University of Calgary
Hossain, Mokarram
0000-0003-0411-944X
University of Calgary
Deppermann, Carsten
University of Hamburg
Lee, Woo Yong
0000-0002-4702-8540
University of Calgary
Haenni, Beat
University of Bern
Surewaard, Bas
University of Calgary
Candinas, Daniel
0000-0002-9924-1749
University of Bern
Kubes, Paul
University of Calgary
Primordial GATA6 macrophages function as extravascular platelets in
sterile injury
Dryad
dataset
2020
FOS: Basic medicine
Swiss National Science Foundation
https://ror.org/00yjd3n13
SNSF P1BEP3_181164
Deutsche Forschungsgemeinschaft
https://ror.org/018mejw64
DE 2654/1-1
Deutsche Forschungsgemeinschaft
https://ror.org/018mejw64
PE 2737/1-1
Natural Sciences and Engineering Research Council
https://ror.org/01h531d29
RGPIN/07191-2019
Banting Postdoctoral Fellowships*
Canadian Institutes of Health Research
https://ror.org/01gavpb45
Canadian Institute of Health Research Fellowships*
Banting Postdoctoral Fellowships
Canadian Institute of Health Research Fellowships
2021-01-11T00:00:00Z
2021-01-11T00:00:00Z
en
926977613 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Most multicellular organisms have a major body cavity that harbors immune
cells. In primordial species like purple sea urchins, these cells perform
phagocytic functions but are also crucial in repairing injuries. In
mammals, the peritoneal cavity contains large numbers of resident GATA6+
macrophages, which may play a similar role. It is unclear how cavity
macrophages suspended in the fluid phase (peritoneal fluid) identify and
migrate towards injuries, however. Here, we show that cavity macrophages
in fluid rapidly form thrombus-like structures in response to injury using
primordial scavenger receptor (SRCR) domains. Aggregates of cavity
macrophages physically sealed injuries and promoted rapid repair of focal
lesions. In iatrogenic surgical situations, these cavity macrophages
formed extensive aggregates that promoted the growth of intra-abdominal
scar tissue termed peritoneal adhesions.
This data package contains a copy of the main data files exactly as used
for the figures in this paper. Please refer to the Material and Methods
section of our manuscript for a detailed description of how this dataset
was collected and processed. In addition to the data shown in our figures,
we provide the raw tif files used for the cell tracking and the respective
R-Scripts used to visualize these tracking results. Data files
are comma-separated values (csv) files except for flow cytometry data
which are stored in a multi-sheet Excel file.
Please start by reading the readme.txt file which contains all necessary
information to use this data set.