10.5061/DRYAD.05510
Wong, Devina
University of British Columbia
Makowska, I. Joanna
University of British Columbia
Weary, Weary M.
Weary, D. M.
University of British Columbia
Data from: Rat aversion to isoflurane versus carbon dioxide
Dryad
dataset
2013
approach-avoidance
Rattus norvegicus
Euthanasia
light/dark box
2013-05-08T18:11:12Z
2013-05-08T18:11:12Z
en
https://doi.org/10.1098/rsbl.2012.1000
13473 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Some experts suggest that sedation of laboratory rodents with isoflurane
before euthanasia with carbon dioxide (CO2) is a humane alternative to
euthanasia with CO2 alone, but little research has compared aversion to
these agents. Albino rats were tested in a light/dark box where they had
the choice between remaining in a dark compartment filling with isoflurane
or CO2, or escaping to a lit compartment. Experiment 1 validated the
procedure by confirming that rats responded to agent and light intensity.
In Experiment 2, 9/16 and 0/16 rats remained in the dark compartment until
recumbent when initially exposed to isoflurane and CO2, respectively. In
Experiment 3, more rats remained in the dark compartment until recumbent
during initial (10/16) versus re-exposure (1/16) to isoflurane. These
results indicate that initial exposure to CO2 is more aversive than
isoflurane, and that re-exposure to isoflurane is more aversive than
initial exposure. We conclude that sedation with isoflurane is a
refinement over euthanasia with CO2 alone for rats that have not been
previously exposed to inhalant anaesthetics.
Time spent in dark compartment during gas exposureThis data was collected
in the UBC Centre for Disease Modelling from 2010-2011. Three experiments
were carried out separately, with each tab in the excel file representing
one experiment. There are two abbreviations: "ISO" represents
isoflurane and "CO2" represents carbon dioxide.Data Deposit.xlsx
Canada
Vancouver
British Columbia