2018
Journal of structural biology 203(3), 281 - 287 (2018). doi:10.1016/j.jsb.2018.05.013
Coiled coils are well described as powerful oligomerization motifs and exhibit a large diversity of functions, including gene regulation, cell division, membrane fusion and drug extrusion. The archaea S-layer originated right-handed coiled coil –RHCC-NT- is characterized by extreme stability and is free of cysteine and histidine moieties. In the current study, we have followed a multidisciplinary approach to investigate the capacity of RHCC-NT to bind a variety of ionic complex metal ions. At the outside of the RHCC-NT, one mercury ion forms an electrostatic interaction with the S-methyl moiety of the single methionine residue present in each coil. We demonstrate that RHCC-NT is reducing and incorporating metallic mercury in the large-sized interior cavities which are lined up along the tetrameric channel.
Published by Elsevier, San Diego, Calif.
10.3204/PUBDB-2019-00829
en
2018
Deutsches Elektronen-Synchrotron, DESY, Hamburg
10.1016/j.jsb.2018.05.013
1047-8477
1095-8657
Journal article
pages 281 - 287
540
Reductive power of the archaea right-handed coiled coil nanotube (RHCC-NT) and incorporation of mercury clusters inside protein cages
McDougall, Matthew
McEleney, Kevin
Francisco, Olga
Trieu, Benchmen
Ogbomo, Efehi Kelly
Tomy, Gregg
Stetefeld, Jörg