10.25750/1995-4301-2015-3-042-061-069A. S. TumanovT. Ya. AshikhminaA. A .Leschenko I. P. PogorelskiyS. A. SharovV. V. TeterinA. G. LazykinG. V. FilimonovA. V. YezhovR. G. PermiakovBio-preparation with a broad spectrum of bio-degradative activity for soil remediation in the chemical weapons destruction plant «Maradykovsky»Theoretical and Applied Ecology2015remediationecotoxicantsorganophosphorus compoundsoilbacteriabiodegradationruDataset1The object of the study is a biological preparation – destructor of organophosphorus compounds, oil and oil products,
created on the basis of the two bacterial strains of Pseudomonas fluorescens ЕC-5-93 and Pseudomonas putida ЕC-8-14.
The biopreparation is designed for soil remediation at the industrial site and adjacent territory of the object for destruction
of chemical weapons «Maradykovsky». The bacteria of both strains are non-pathogenic, biocompatible, ecologically safe,
stable on the grounds of biodegradation of ecotoxicants, unpretentious for nutritional needs, technological, do not persist
in the environment in the absence of a substrate for degradation. The technology of production of the biopreparation
is designed. Ready to use freeze-dried biological preparation is a porous mass of light-yellow color without inclusions.
The aim of the study is to expand the range of degradative potential of the biopreparation due to the additional
introduction into its structure of the bacterial strain Pseudomonas putida ЕC-8-14 – effective destructor of oil pollutions
of soil. The activity to oil degradation of the biopreparation obtained according to the technology developed has been
investigated in experiments in vitro by culturing the microorganisms included in the biopreparation in mineral medium
with oil. Simulation of natural conditions of soil remediation and the study of the ability of the biopreparation to degradation
of glyphosate was carried out on a test stand with systems for air-conditioning, monitoring, recording and maintenance
of technological parameters of recultivation. Efficiency of ecotoxicants degradation was assessed by CG-CM spectrometry
with mass-selective detection. The high activity of the microorganisms included in the biopreparation, to degradation
of petroleum hydrocarbons and ecotoxicants is shown. During the analytical determination of glyphosate in soil it was
found that when the initial content of glyphosate was 53,6 mg/kg, to 12 days of experiment the amount of ecotoxicants
decreased to the level of 0,15 mg/kg, which is lower than the initial content in 357 times and the MPC(0,5 mg/kg).
The results of the study indicate the possibility of the practical use of the biopreparation-destructor degradative
potential in the course of bioremediation of soil and cleaning it from toxicants.