10.25402/FSOA.C.4441841.V1
Frøydis Sved Skottvoll
Henriette Engen Berg
Kamilla Bjørseth
Kaja Lund
Norbert Roos
Sara Bekhradnia
Bernd Thiede
Cecilie Sandberg
Einar Osland Vik-Mo
Hanne Roberg-Larsen
Bo Nyström
Elsa Lundanes
Steven Ray Wilson
Supplementary Material for Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations: supplementary material
<div><b>Lay abstract</b></div><div><br></div><div>Exosomes are small vesicles that are released from biological cells. Exosomes are viewed as being tools for intracellular communication, and there is evidence that cancer cells can release exosomes to, for example, enable metastasis. Exosomes may be very useful for diagnostics, as the detection and characterization of exosomes may allow disease detection at early stages. However, exosomes must be isolated from blood or other biomaterials prior to analysis. This can be a challenging task, and we have here taken a critical look at two familiar isolation techniques, using samples from breast cancer cell lines and brain cancer cells (glioblastoma). We find that the two approaches have comparable performance, and contain protein biomarkers of their cells of origin. However, the isolated exosomes can contain contaminations, which may cloud the analysis in clinical settings. Hence, there is room for new approaches for the isolation of exosomes.</div><div><br></div><div><div><b>1. Supplemental DLS data</b></div><div>All figures are correlation functions from DLS analyses of isolated exosome samples. Table 1 shows an overview of the figure number related to the sample code of interest, together with the measured hydrodynamic radius in the samples.</div><div><b>1.2 Table 1.</b> Figure number of the related correlation function to the different sample codes, together with the measured hydrodynamic radius (nm).</div><div><b>1.3 Figure 1.</b> Correlation function of BC UC replicate 1.</div><div><b>1.4 Figure 2. </b>Correlation function of BC UC replicate 2.</div><div><b>1.5 Figure 3.</b> Correlation function of BC UC replicate 3.</div><div><b>1.6 Figure 4.</b> Correlation function of BC kit replicate 1.</div><div><b>1.7 Figure 5. </b>Correlation function of BC kit replicate 2.</div><div><b>1.8 Figure 6. </b>Correlation function of GBM UC replicate 1.</div><div><b>1.9 Figure 7. </b>Correlation function of GBM kit replicate 1.</div><div><br></div><div><b>2. Supplemental Methods</b> </div><div><br></div><div><b>3. Supplementary Proteins </b></div><div><b><br></b></div><div><b>4. Supplementary Western Blots</b></div></div>
60101 Analytical Biochemistry
60108 Protein Trafficking
110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Figshare
2019
2019-03-20
2019-03-20
Collection
10.4155/fsoa-2018-0088
10.25402/FSOA.c.4441841
CC BY 4.0