10.25338/B86D27
Luecke, David
0000-0001-5309-9153
University of California, Davis
Drosophila enhancer adult bristle expression assay using UAS sv-RNAi
Dryad
dataset
2021
FOS: Biological sciences
2021-08-12T00:00:00Z
2021-08-12T00:00:00Z
en
https://doi.org/10.5281/zenodo.5139174
https://doi.org/10.5281/zenodo.5192425
42209 bytes
4
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Data from manuscript "Sex-specific evolution of a Drosophila sensory
system via interacting cis- and trans-regulatory changes" by D
Luecke, G Rice, and A Kopp. This study examines the regulation of
expression for the chemosensory bristle specifier gene Pox neuro, which
has expanded expression into ancestrally mechanosensory bristles in
male D. prolongata. Flourescent assays show qualitativetly expanded
expression driven by the D. prolongata allele relative to enhancers from
sister species in the developing pupal leg. Adult bristle disruption
assays using shavenoid RNAi show quantitative expression expansion by the
D. prolongata allele into specific mechanosensory bristles on the
dorso-anterior leg of the first tarsi.
Pox neuro enhacer regions were cloned into Gal4 reporter vector pBPGUw and
inserted site-specifically into the attP2 docking site. Homozygous
reporter-Gal4 flies were crossed to UAS-dcr2; UAS-svRNAi flies. T1 legs
from the F1 of this cross were mounted in PVA clearing media and examined
under light microscopy. Individual bristles were scored as 0 for missing,
0.5 for truncated, and 1 for intact. Comparisons between number of
effected bristle per species enhancer allele were done by pairwise t test,
with bonferonni correction for multiple tests.
Bristle identities are based on the included first tarsus diagram,
modified from Tokunagu 1962. Missing values represent intact bristles, and
are treated as such (1s) by the accompanying R script.