10.21979/N9/M2W2YL
Hoo, Regina May Ling
(Nanyang Technological University)
Transcriptome profiling of Plasmodium falciparum parasites from controlled human malaria infection studies
DR-NTU (Data)
2019
The transcriptional landscape of Plasmodium falciparum clinical isolates varies according to strain, mosquito bites, disease severity and clinical history. These confounding variations can be potentially overcome with single-strain parasites derived from controlled-human malaria infection (CHMI) studies. Here, we deconvolute the transcriptome complexity using CHMI-derived parasites from volunteers receiving the same PfPSZ dosage (Sanaria® PfSPZ Challenge). While we observe minimal interindividual transcriptional variation, there are clear deviations between volunteer groups receiving different PfSPZ doses and the pre-mosquito strain used for PfSPZ generation. Gene cluster associated with malaria pathogenesis, immune evasion and transmission show significant transcriptional variability between the CHMI-derived parasites after transition from mosquito to human infection. The extent of this transcriptional variation signature cluster was also seen in P. falciparum parasites directly isolated from acute clinical infections, implying the potential use of transcriptomics data from CHMI-derived parasites to support understanding of parasite biology after mosquito passage and human infection.
Hoo, Regina May Ling
(Nanyang Technological University)