10.21958/PHENOTYPE:361
Daniela, Ristova
Mean(R) IAA
Daniela Ristova
2019
Sept. 3, 2018, 1:04 p.m.
Jan. 16, 2019, 11:09 a.m.
en-us
Phenotype
10.21958/study:28
30030851
190 values
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application/json
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1
After 3 days of treatment, plates were scanned with CCD flatbed scanners (EPSON Perfection V600 Photo, Seiko Epson, Nagano, Japan), and images used to quantify root parameters with FIJI, by using the tool ‘segmented line’ (Schindelin et al., 2012) as described in Ristova and Busch (2017). In particular, we quantified: primary root length on day 10 (P), growth rate of P after treatment (P2), branching zone or the length of P between the first and last visible lateral root (R), average lateral root length (LRL), and visible lateral root number (LR.No).
Seeds were surface sterilized in a desiccator using 100 ml of household bleach and 3.5 ml of 37% HCl for 1 h. The seeds were stratified in water at 4°C in the dark for 3 days, and were sown on square Petri dishes containing 57 ml of growth medium. The control growth medium (before transfer) consisted of one‐fifth‐strength MS medium with MES buffer (Ducheva Biochemie, Haarlem, The Netherlands), 1% sucrose, 0.8% agar (Ducheva Biochemie), and adjusted to pH 5.7.