10.21958/PHENOTYPE:1282
Robin, Lardon
shoot primordia 21d protocol a
Robin Lardon
2020
Aug. 18, 2020, 9:33 a.m.
Aug. 20, 2020, 5:14 p.m.
en-us
Phenotype
10.21958/study:80
1711 values
text/csv
application/json
application/plink
1
Manual count of regenerated shoot primordia from root explants after 21 days on SIM following protocol a. Shoot primordia were defined as dome-shaped outgrowths with a purple or green colour and clearly organized cellular patterning, indicated by a smooth surface.
Protocol a: 7-mm-long apical root segments were excised from 7-day-old seedlings, grown under cool white fluorescent tungsten tubes (70 µM m-2 s-1; 14/10 h light/dark regime) at 21 °C. Next, explants were preincubated on callus induction medium (CIM; B5 supplemented with 2.2 µM 2,4-dichlorophenoxy acetic acid and 0.2 µM kinetin) for 4 days, before being transferred to shoot induction medium (SIM; B5 supplemented with 25 µM 2-isopentenyl adenine and 0.86 µM 3-indole acetic acid).