10.17867/10000114
Timing of gene expression in a cell-fate decision system
Aymoz Delphine
Aymoz
0000-0002-4903-6229
University of Lausanne
Solé Carme
Solé
0000-0003-4543-7401
Universitat Pompeu Fabra
Pierre Jean-Jerrold
Pierre
University of Lausanne
Schmitt Marta
Schmitt
University of Lausanne
de Nadal Eulalia
de Nadal
0000-0003-0039-5607
Universitat Pompeu Fabra
Posas Francesc
Posas
0000-0002-4164-7076
Universitat Pompeu Fabra
Pelet Serge
Pelet
0000-0002-0245-049X
University of Lausanne
University of Dundee
2018
Image
morphogens
cell-fate
gene expression
transcription
mating pathway
budding yeast
2018
2018-03-29
https://doi.org/10.1101/197103
During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the physiological context of mating. In both conditions, we observed striking differences in the timing of induction of mating-responsive promoters. Biochemical analyses and generation of synthetic promoter variants demonstrated how the interplay between transcription factor binding and nucleosomes contribute to determine the kinetics of transcription in a simplified cell-fate decision system.
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