10.17867/10000110
Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy
Marie Held
0000-0003-0118-5898
Institute of Integrative Biology, University of Liverpool
University of Dundee
2017
Image
Renal re-aggregation, organoid, light sheet fluorescence microscopy, nephron, differentiation, glomeruli, podocytes, live imaging, cell tracking
Ilaria Santeramo
Institute of Translational Medicine, University of Liverpool
Bettina Wilm
0000-0002-9245-993X
Institute of Translational Medicine, University of Liverpool
Patricia Murray
0000-0002-9245-993X
Institute of Translational Medicine, University of Liverpool
Raphaël Lévy
0000-0001-5728-0531
Institute of Integrative Biology, University of Liverpool
2017-11-08
We have adapted the mouse kidney rudiment assay to generate renal organoids. We have immunostained endpoint fixed organoids and E13.5 embryonic kidneys and imaged them with a light sheet fluorescent microscope. The data generated for this manuscript is made accessible here and includes immunofluorescence staining after end point fixation revealing that organotypic structures develop within the organoids. Furthermore, we have performed an assay to ascertain tubular functionality. Following, we have imaged organoids containing Wt1-GFP knock-in cells live on the light sheet microscope to evaluate the ability to follow cells long-term and generate previously unavailable quantitative tracking data.
En
CC-BY 4.0