10.1594/PANGAEA.881803
Wermter, Felizitas Charlotte
Felizitas Charlotte
Wermter
0000-0002-7933-8447
Mitschke, Nico
Nico
Mitschke
0000-0002-1043-7199
Bock, Christian
Christian
Bock
0000-0003-0052-3090
Dreher, Wolfgang
Wolfgang
Dreher
Temperature dependence of 1H NMR chemical shifts and its influence on estimated metabolite concentrations, link to supplementary material
PANGAEA
2017
en
Supplementary Dataset
10.1007/s10334-017-0642-z
30.4 MBytes
application/zip
Creative Commons Attribution 3.0 Unported
Objectives: Temperature dependent chemical shifts of important brain metabolites measured by localised 1H MRS were investigated to test how the use of incorrect prior knowledge on chemical shifts impairs the quantification of metabolite concentrations.
Materials and methods: Phantom measurements on solutions containing 11 metabolites were performed on a 7 T scanner between 1 and 43 °C. The temperature dependence of the chemical shift differences was fitted by a linear model. Spectra were simulated for different temperatures and analysed by the AQSES program (jMRUI 5.2) using model functions with chemical shift values for 37 °C.
Results: Large differences in the temperature dependence of the chemical shift differences were determined with a maximum slope of about ±7.5 × 10-4 ppm/K. For 32-40 °C, only minor quantification errors resulted from using incorrect chemical shifts, with the exception of Cr and PCr. For 1-10 °C considerable quantification errors occurred if the temperature dependence of the chemical shifts was neglected.
Conclusion: If 1H MRS measurements are not performed at 37°C, for which the published chemical shift values have been determined, the temperature dependence of chemical shifts should be considered to avoid systematic quantification errors, particularly for measurements on animal models at lower temperatures.
Supplement to: Wermter, Felizitas Charlotte; Mitschke, Nico; Bock, Christian; Dreher, Wolfgang (2017): Temperature dependence of 1H NMR chemical shifts and its influence on estimated metabolite concentrations. Magnetic Resonance Materials in Physics, Biology and Medicine, 12 pp