10.15480/882.4485
Hofmann, Sebastian
Sebastian
Hofmann
0000-0002-4312-7402
1233992279
Weiland, Christian
Christian
Weiland
0000-0003-3321-987X
1263165389
Fitschen, Jürgen
Jürgen
Fitschen
0000-0002-1418-933X
1201611253
Kameke, Alexandra von
Alexandra von
Kameke
0000-0002-1913-774X
1209512920
Hoffmann, Marko
Marko
Hoffmann
1032814101
Schlüter, Michael
Michael
Schlüter
0000-0001-5969-2150
1092203567
Lagrangian sensors in a stirred tank reactor: comparing trajectories from 4D-Particle Tracking Velocimetry and Lattice-Boltzmann simulations
Elsevier
2022
4D-Particle Tracking Velocimetry
CFD
Flow-following
Lagrangian sensor particle tracking
Lattice-Boltzmann
Trajectories
Ingenieurwissenschaften
TUHH Universitätsbibliothek
TUHH Universitätsbibliothek
2022-07-21
2022-07-21
2022-06-17
en
Journal Article
Chemical Engineering Journal 449: 137549 (2022-12-01)
http://hdl.handle.net/11420/13239
10.15480/882.4485
10.1016/j.cej.2022.137549
https://creativecommons.org/licenses/by/4.0/
In this study, three-dimensional flow measurements by means of 4D-Particle Tracking Velocimetry (4D-PTV) are carried out in a laboratory-scale 3 L stirred tank reactor in order to investigate the flow-following behavior of two different inertial particle types, Polyethylene (PE) particles and alginate beads, at different impeller frequencies. Applied particles mimic Lagrangian sensor particles, which are intended to determine process parameters such as oxygen concentration at their corresponding position inside a bioreactor. Accompanying Lattice-Boltzmann Large Eddy Simulations (LB LES) provide additional information about the fluid flow and the difference in the trajectories between inertial and non-inertial particles. The data acquired from LB LES is validated with the experimental data by means of a Lagrangian and a Eulerian approach. In their tail, the probability distributions show higher Lagrangian velocities and accelerations for 4D-PTV data compared to LB LES data. Time-averaged Eulerian data is utilized to determine particle Reynolds numbers lower than 200. The Stokes number distributions show 10-fold higher values for the alginate beads than for PE particles, however, both particle types do not sufficiently meet the criterion of a flow-following Stokes number of St≤0.01. Generally, time-averaged results from LB LES are in good accordance to the 4D-PTV data. From the LB LES, a theoretical, maximum particle diameter of approx. 20 μm is determined, which meets the criterion of St≤0.01 throughout the reactor. This result implies that with current sensor particle technology it is not possible to meet the flow-following behavior and depict the lifelines of cells during a cultivation process. Therefore, further research is necessary to understand particle trajectories and to translate them into lifelines of cells.
Deutsche Forschungsgemeinschaft (DFG)
SCHL 617/19-1
SPP 2170: Teilprojekt Experimentelle Multiskalenanalyse und Simulation von 'lifelines' in Bioreaktoren um deren Einfluss auf die Kultivierung von Chinese Hamster Ovary (CHO) Zellen zu untersuchen
1873-3212
Chemical engineering journal
2022
Elsevier