Myosin II-mediated cell shape changes and cell intercalation contribute to primitive streak formation - Rozbicki, Emil; Manli Chuai; Karjalainen, Antti I.; Feifei Song; Sang, Helen M.; Martin, René; Hans-Joachim Knölker; MacDonald, Michael P.; Weijer, Cornelis J. (University of Dundee, 2015)
The dataset shown represents a timelapse sequence showing cell and tissue dynamics during primitive streak formation in chick embryo. The data were acquired using Myr-GFP embryos and a custom built light-sheet microscope. The sequence shows the developmental period covering the time interval from the freshly laid egg (stage EGXIII), prior to the onset of tissue movement until the end of the formation of the primitive streak (Stage HH4). Shown here is only one of the x,y planes from a 3D stack of 100 x,y,z planes that was used for further quantitative analysis of cell and tissue dynamics. The individual results of these analyses are presented as the overlays and are described in the Channel boxes and the accompanying '.txt' file. The dataset was acquired using a custom light-sheet microscope designed and constructed at the University of Dundee in collaboration between Dr Michael McDonald and Prof. Cornelis Weijer. This work was funded by Biotechnology and Biological Sciences Research Council. The images can be viewed directly on this website using OMERO.web viewer or downloaded and opened using any program that supports the '.tif' format. To view the sequence with the overlays we recommend to open the '.pattern' file with Bioformats or Fiji with the Bioformats importer installed. The dataset accompanies data presented in the Nature Cell Biology manuscript 10.1038/ncb3138. The sample preparation protocol is described in Nature Protocol Exchange 10.1038/protex.2015.012.