10.1184/R1/6096452.V1
Adam Feinberg
Crystal M. Ripplinger
Peter van der Meer
Sean P. Sheehy
Ibrahim Domian
Kenneth R. Chien
Kevin Kit Parker
Functional differences in engineered myocardium from embryonic stem cell-derived versus neonatal cardiomyocytes.
<p>Stem cell-derived cardiomyocytes represent unique tools for cell- and tissue-based regenerative therapies, drug discovery and safety, and studies of fundamental heart-failure mechanisms. However, the degree to which stem cell-derived cardiomyocytes compare to mature cardiomyocytes is often debated. We reasoned that physiological metrics of engineered cardiac tissues offer a means of comparison. We built laminar myocardium engineered from cardiomyocytes that were differentiated from mouse embryonic stem cell-derived cardiac progenitors or harvested directly from neonatal mouse ventricles, and compared their anatomy and physiology in vitro. Tissues assembled from progenitor-derived myocytes and neonate myocytes demonstrated similar cytoskeletal architectures but different gap junction organization and electromechanical properties. Progenitor-derived myocardium had significantly less contractile stress and slower longitudinal conduction velocity than neonate-derived myocardium, indicating that the developmental state of the cardiomyocytes affects the electromechanical function of the resultant engineered tissue. These data suggest a need to establish performance metrics for future stem cell applications.</p>
90399 Biomedical Engineering not elsewhere classified
Carnegie Mellon University
2013
2018-04-05
2018-04-05
Journal contribution
2158949 Bytes
10.1184/R1/6096452
CC BY-NC-ND 4.0