{
"id": "https://doi.org/10.7490/f1000research.1000208.1",
"doi": "10.7490/F1000RESEARCH.1000208.1",
"url": "http://f1000research.com/posters/4-233",
"types": {
"ris": "GEN",
"bibtex": "misc",
"citeproc": "article",
"schemaOrg": "CreativeWork",
"resourceTypeGeneral": "Other"
},
"creators": [
{
"name": "Liénard, Emmanuel",
"nameType": "Personal",
"givenName": "Emmanuel",
"familyName": "Liénard",
"affiliation": []
}
],
"titles": [
{
"title": "Experimental infections of rabbits with proliferative and latent stages of Besnoitia besnoiti"
}
],
"publisher": {
"name": "F1000Research"
},
"container": {},
"subjects": [],
"contributors": [],
"dates": [
{
"date": "2015",
"dateType": "Issued"
}
],
"publicationYear": 2015,
"identifiers": [],
"sizes": [],
"formats": [],
"rightsList": [],
"descriptions": [
{
"description": "Cattle besnoitiosis due to Besnoitia besnoiti is in expansion in Europe since the end of the twentieth century. This disease is responsible for severe economic losses in newly infected herd. Unfortunately, no treatment is available and no vaccine is currently labelled in Europe. The complete clinical picture following the artificial transmission of the parasite to cattle remains difficult and random. Rabbit Oryctolagus cuniculus has been found to be more or less susceptible to this parasite experimentally. This species can exhibit all the clinical signs observed in cattle. The adaptation of B. besnoiti to the rabbit and its maintenance could offer a new, easiest to handle and cheapest model of investigation to this disease. This experimental study has compared the virulence between tachyzoites and bradyzoites of B. besnoiti on rabbits. Eighteen New Zealand rabbits aged from six months to four years old were allocated into three groups of six animals. The control group (group C) received subcutaneous injection of 30 µl of ovalbumin at the right flank. 2.106 tachyzoites of B. besnoiti cultivated on Vero cells, at the passage 125 were subcutaneously injected at the right flank of the group “Tachyzoites” (group T). Bradyzoites of B. besnoiti were taken from cutaneous cysts of chronically infected cow. 2.106 bradyzoites were then injected subcutaneously at the right flank of the group “Bradyzoites” (group B). Clinical follow-up and serological survey were performed during ten weeks until the euthanasia. Only the group B has exhibited an acute stage of the disease with febrile syndrome above 40°C from day 8 to day 11 after the injection with positive qPCR in blood. Twenty-five samples of skin and organs were investigated for detection of parasite DNA by qPCR and parasites by histology. Cysts of B. besnoiti were found only on the group B in some skin samples and organs corresponding to positive qPCR results (Ct values < 30). These results suggest a high virulence of bradyzoites in rabbits. The proposed model is technically simple to perform and highly efficient to reproduce the disease in rabbit. It could be used for the in vivo assessment of efficacy of vaccine or treatment against cattle besnoitiosis.
",
"descriptionType": "Other"
}
],
"geoLocations": [],
"fundingReferences": [],
"relatedIdentifiers": [],
"providerId": "zwfe",
"clientId": "bl.f1000r",
"agency": "datacite",
"state": "findable"
}