{
"id": "https://doi.org/10.6084/m9.figshare.97903",
"doi": "10.6084/M9.FIGSHARE.97903",
"url": "https://figshare.com/articles/journal_contribution/HIGH_PROLIFERATION_AND_NORMAL_APOPTOSIS_ARE_THE_KINETIC_HALLMARKS_OF_CUTANEOUS_MERKEL_CELL_CARCINOMAS/97903",
"types": {
"ris": "RPRT",
"bibtex": "article",
"citeproc": "article-journal",
"schemaOrg": "ScholarlyArticle",
"resourceType": "Journal contribution",
"resourceTypeGeneral": "Text"
},
"creators": [
{
"name": "Diaz-Cano, Salvador J.",
"nameType": "Personal",
"givenName": "Salvador J.",
"familyName": "Diaz-Cano",
"affiliation": [],
"nameIdentifiers": [
{
"schemeUri": "https://orcid.org",
"nameIdentifier": "https://orcid.org/0000-0003-1245-2859",
"nameIdentifierScheme": "ORCID"
}
]
},
{
"name": "A Blanes",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "I Hierro",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "J Rubio",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "A Martinez",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "JJ Sanchez-Carrillo",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "A Matilla",
"affiliation": [],
"nameIdentifiers": []
}
],
"titles": [
{
"title": "HIGH PROLIFERATION AND NORMAL APOPTOSIS ARE THE KINETIC HALLMARKS OF CUTANEOUS MERKEL CELL CARCINOMAS"
}
],
"publisher": {
"name": "figshare"
},
"container": {},
"subjects": [
{
"subject": "Medicine"
},
{
"subject": "Cancer"
}
],
"contributors": [],
"dates": [
{
"date": "2012-11-22",
"dateType": "Created"
},
{
"date": "2012-11-23",
"dateType": "Updated"
},
{
"date": "2012",
"dateType": "Issued"
}
],
"publicationYear": 2012,
"identifiers": [],
"sizes": [
"132886 Bytes"
],
"formats": [],
"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
"schemeUri": "https://spdx.org/licenses/",
"rightsIdentifier": "cc-by-4.0",
"rightsIdentifierScheme": "SPDX"
}
],
"descriptions": [
{
"description": "Background: Merkel cell carcinomas (MCCs) are unusual cutaneous neoplasms that reveal both epithelial and neuroendocrine differentiation. No detailed analysis of cell kinetics (proliferation and apoptosis) by topographic compartments is available to date. Methods: All MCCs were required to express at least two epithelial and two neural markers from a panel including cytokeratin cocktail AEl -AE3, cytokeratin 20, synaptophysin, chromogranin A, neurofilament protein, and neuron-specific enolase, along with consistent ultrastructural findings. We selected 19 MCCs to evaluate mitotic figure (MF) counting, Ki-67 index, and apoptosis index based on the in situ end labeling (ISEL) of fragmented DNA using digoxigenin-labeled dUTP and Escherichia coli DNA polymerase I (Klenow fragment). At least 50 high-power fields were screened per topographic compartment (superficial or papillary dermis, and deep or reticular dermis), recording average and standard deviation for each variable. Variables were statistically compared in each tumor compartment using analysis of variance and Student t-test (significant if P<0.05.
Results: MCCs revealed high cell density (over 425 cell/HPF) and no statistical differences for theproliferation-markers by topographic compartments. Apoptosis showed lower values in the deep compartment, but only significant for the standard deviation of ISEL index (P=0.0074).
Conclusions: Homogeneously distributed high proliferation defines MCCs. Apoptosis follows the distribution pattern of proliferation in superficial compartments, whereas in deep compartments the apoptosis distribution is less variable and independent from proliferation.",
"descriptionType": "Abstract"
}
],
"geoLocations": [],
"fundingReferences": [],
"relatedIdentifiers": [],
"schemaVersion": "http://datacite.org/schema/kernel-4",
"providerId": "otjm",
"clientId": "figshare.ars",
"agency": "datacite",
"state": "findable"
}