{
"id": "https://doi.org/10.6084/m9.figshare.5918590",
"doi": "10.6084/M9.FIGSHARE.5918590",
"url": "https://karger.figshare.com/articles/Supplementary_Material_for_Neuroinflammation_in_Response_to_Intracerebral_Injections_of_Different_HMGB1_Redox_Isoforms/5918590",
"types": {
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"creators": [
{
"name": "Aucott, H.",
"nameType": "Personal",
"givenName": "H.",
"familyName": "Aucott",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Lundberg, J.",
"nameType": "Personal",
"givenName": "J.",
"familyName": "Lundberg",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Salo, H.",
"nameType": "Personal",
"givenName": "H.",
"familyName": "Salo",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Klevenvall, L.",
"nameType": "Personal",
"givenName": "L.",
"familyName": "Klevenvall",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Damberg, P.",
"nameType": "Personal",
"givenName": "P.",
"familyName": "Damberg",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Ottosson, L.",
"nameType": "Personal",
"givenName": "L.",
"familyName": "Ottosson",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Andersson, U.",
"nameType": "Personal",
"givenName": "U.",
"familyName": "Andersson",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Holmin, S.",
"nameType": "Personal",
"givenName": "S.",
"familyName": "Holmin",
"affiliation": [],
"nameIdentifiers": []
},
{
"name": "Erlandsson Harris, H.",
"nameType": "Personal",
"givenName": "H.",
"familyName": "Erlandsson Harris",
"affiliation": [],
"nameIdentifiers": []
}
],
"titles": [
{
"title": "Supplementary Material for: Neuroinflammation in Response to Intracerebral Injections of Different HMGB1 Redox Isoforms"
}
],
"publisher": {
"name": "Karger Publishers"
},
"container": {},
"subjects": [
{
"subject": "Medicine"
}
],
"contributors": [],
"dates": [
{
"date": "2018-02-23",
"dateType": "Created"
},
{
"date": "2018-02-23",
"dateType": "Updated"
},
{
"date": "2018",
"dateType": "Issued"
}
],
"publicationYear": 2018,
"identifiers": [],
"sizes": [
"380782 Bytes"
],
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"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
"schemeUri": "https://spdx.org/licenses/",
"rightsIdentifier": "cc-by-4.0",
"rightsIdentifierScheme": "SPDX"
}
],
"descriptions": [
{
"description": "Background: Neuroinflammation triggered by infection or trauma is the cause of central nervous system dysfunction. High-mobility group box 1 protein (HMGB1), released from stressed and dying brain cells, is a potent neuroinflammatory mediator. The proinflammatory functions of HMGB1 are tightly regulated by post-translational redox modifications, and we here investigated detailed neuroinflammatory responses induced by the individual redox isoforms. Methods: Male Dark Agouti rats received a stereotactic injection of saline, lipopolysaccharide, disulfide HMGB1, or fully reduced HMGB1, and were accessed for blood-brain barrier modifications using magnetic resonance imaging (MRI) and inflammatory responses by immunohistochemistry. Results and Conclusions: Significant blood-brain barrier disruption appeared 24 h after injection of lipopolysaccharide, disulfide HMGB1, or fully reduced HMGB1 compared to controls, as assessed in post-gadolinium T1-weighted MRI images and confirmed by increased uptake of FITC-conjugated dextran. Immunohistochemistry revealed that both HMGB1 isoforms also induced a local production of IL-1β. Additionally, disulfide HMGB1 increased major histocompatibility complex class II expression and apoptosis. Together, the results demonstrate that extracellular, cerebral HMGB1 causes significant blood-brain barrier disruption in a redox-independent manner and activates several components of neuroinflammation. Blocking HMGB1 might potentially improve clinical outcome in conditions such as stroke and traumatic brain injury.",
"descriptionType": "Abstract"
}
],
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{
"relationType": "IsSupplementTo",
"relatedIdentifier": "10.1159/000487056",
"relatedIdentifierType": "DOI"
}
],
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"agency": "datacite",
"state": "findable"
}