{
"id": "https://doi.org/10.6084/m9.figshare.20260974.v1",
"doi": "10.6084/M9.FIGSHARE.20260974.V1",
"url": "https://tandf.figshare.com/articles/journal_contribution/Construction_of_a_membrane_yeast_two-hybrid_library_and_its_application_in_identification_of_PsARRO-1-interacting_proteins_in_tree_peony/20260974/1",
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"creators": [
{
"name": "Wang, Huiqin",
"givenName": "Huiqin",
"familyName": "Wang"
},
{
"name": "Wang, Zheng",
"givenName": "Zheng",
"familyName": "Wang"
},
{
"name": "Lou, Xueyuan",
"givenName": "Xueyuan",
"familyName": "Lou"
},
{
"name": "He, Songlin",
"givenName": "Songlin",
"familyName": "He"
}
],
"titles": [
{
"title": "Construction of a membrane yeast two-hybrid library and its application in identification of PsARRO-1-interacting proteins in tree peony"
}
],
"publisher": {
"name": "Taylor & Francis"
},
"container": {},
"subjects": [
{
"subject": "Biochemistry"
},
{
"subject": "Medicine"
},
{
"subject": "Microbiology"
},
{
"subject": "FOS: Biological sciences",
"schemeUri": "http://www.oecd.org/science/inno/38235147.pdf",
"subjectScheme": "Fields of Science and Technology (FOS)"
},
{
"subject": "Cell Biology"
},
{
"subject": "Molecular Biology"
},
{
"subject": "Biotechnology"
},
{
"subject": "Ecology"
},
{
"subject": "Biological Sciences not elsewhere classified"
},
{
"subject": "Plant Biology"
}
],
"contributors": [],
"dates": [
{
"date": "2022-07-07",
"dateType": "Created"
},
{
"date": "2024-02-19",
"dateType": "Updated"
},
{
"date": "2022",
"dateType": "Issued"
}
],
"publicationYear": 2022,
"identifiers": [],
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"288886 Bytes"
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"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
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"rightsIdentifier": "cc-by-4.0",
"rightsIdentifierScheme": "SPDX"
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],
"descriptions": [
{
"description": "To investigate the molecular mechanism of the ADVENTITIOUS ROOTING RELATED OXYGENASE 1 (ARRO-1) gene in adventitious rooting of tree peony, the PsARRO-1 gene was isolated from Paeonia ostii. Subsequently, a yeast two-hybrid (Y2H) cDNA library using the split-ubiquitin yeast membrane system was constructed, the insert fragments lengths of pPR3-N membrane Y2H library were mainly concentrated between 500 and 3,000 bp, and the library capacity reached 1.3 × 107 CFUs, with an efficiency of recombinant of 100%, which met the requirements for subsequent screening of PsARRO-1-interacting proteins. Finally, 15 candidate PsARRO-1-interacting proteins were screened out by pBT3SUC-ARRO, among them potassium transporter 2, cytochrome b5 isoform E, and high mobility group B protein 3 (HMGB3) might be involved in the formation of roots. Both direct Y2H and bimolecular fluorescence complementation assays confirmed that PsARRO-1 interacted with PsHMGB3, which shared high identity with EgHMGB3 and MiHMGB3. The expression of PsHMGB3 was significantly up-regulated in the root outgrowth stage, and the overexpression of PsHMGB3 in Arabidopsis resulted in lengthened primary roots, more lateral roots. In short, the Y2H library provides a large number of useful resources for functional genomics research on roots of tree peony.",
"descriptionType": "Abstract"
}
],
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"relatedIdentifier": "10.1080/14620316.2022.2092561",
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