{
"id": "https://doi.org/10.6084/m9.figshare.19145743",
"doi": "10.6084/M9.FIGSHARE.19145743",
"url": "https://tandf.figshare.com/articles/figure/Exosomes_from_miR-374a-5p-modified_mesenchymal_stem_cells_inhibit_the_progression_of_renal_fibrosis_by_regulating_MAPK6_MK5_YAP_axis/19145743",
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"creators": [
{
"name": "Liang, Mingzhu",
"givenName": "Mingzhu",
"familyName": "Liang"
},
{
"name": "Zhang, Di",
"givenName": "Di",
"familyName": "Zhang"
},
{
"name": "Zheng, Danna",
"givenName": "Danna",
"familyName": "Zheng"
},
{
"name": "He, Wenfang",
"givenName": "Wenfang",
"familyName": "He"
},
{
"name": "Jin, Juan",
"givenName": "Juan",
"familyName": "Jin"
}
],
"titles": [
{
"title": "Exosomes from miR-374a-5p-modified mesenchymal stem cells inhibit the progression of renal fibrosis by regulating MAPK6/MK5/YAP axis"
}
],
"publisher": {
"name": "Taylor & Francis"
},
"container": {},
"subjects": [
{
"subject": "Biochemistry"
},
{
"subject": "Medicine"
},
{
"subject": "Cell Biology"
},
{
"subject": "Genetics"
},
{
"subject": "FOS: Biological sciences",
"schemeUri": "http://www.oecd.org/science/inno/38235147.pdf",
"subjectScheme": "Fields of Science and Technology (FOS)"
},
{
"subject": "Molecular Biology"
},
{
"subject": "Pharmacology"
},
{
"subject": "Chemical Sciences not elsewhere classified"
},
{
"subject": "Immunology"
},
{
"subject": "FOS: Clinical medicine",
"schemeUri": "http://www.oecd.org/science/inno/38235147.pdf",
"subjectScheme": "Fields of Science and Technology (FOS)"
},
{
"subject": "Biological Sciences not elsewhere classified"
},
{
"subject": "Developmental Biology"
},
{
"subject": "Marine Biology"
},
{
"subject": "Cancer"
},
{
"subject": "Hematology"
},
{
"subject": "Computational Biology"
}
],
"contributors": [],
"dates": [
{
"date": "2022-02-09",
"dateType": "Created"
},
{
"date": "2024-03-21",
"dateType": "Updated"
},
{
"date": "2022",
"dateType": "Issued"
}
],
"publicationYear": 2022,
"identifiers": [],
"sizes": [
"88884 Bytes"
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"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
"schemeUri": "https://spdx.org/licenses/",
"rightsIdentifier": "cc-by-4.0",
"rightsIdentifierScheme": "SPDX"
}
],
"descriptions": [
{
"description": "Chronic kidney disease (CKD) in clinical is defined as a gradual loss of kidney function for more than 3 months. The pathologic course of CKD is characterized by extensive renal fibrosis; thus, preventing renal fibrosis is vital for the treatment of CKD. It has been reported that microRNA (miR)-374a-5p was under-expressed in renal venous blood samples from patients with CKD. In addition, it exhibited anti-apoptotic effects in renal tissues suggesting that miR-374a-5p may play an important role in CKD. However, it is not clear whether miR-374a-5p could be delivered to renal cells by exosomes and exerts anti-renal fibrosis effects. To mimic renal fibrosis in vitro, human renal tubular epithelial cell lines (HK-2 cells) were treated by transforming growth factor-β (TGF-β) 1. Reverse transcription-quantitative polymerase-chain reaction (RT-qPCR) or Western blot was carried out to evaluate the mechanism by which miR-374a-5p regulated the development of renal fibrosis. Next, exosomes were isolated using with ultracentrifugation method, and the relationship between miR-374a-5p and MAPK6 was evaluated using dual-Luciferase a reporter assay system. The results indicated TGF-β1 significantly down-regulated the expression of miR-374a-5p in HK-2 cells and miR-374a-5p agomir remarkably inhibited the progression of fibrosis in vitro. In addition, exosomal miR-374a-5p could be internalized by HK-2 cells and obviously enhanced the level of miR-374a-5p in HK-2 cells. Furthermore, exosomal miR-374a-5p prevented the progression of renal fibrosis in vivo by regulating MAPK6/MK5/YAP axis. In conclusion, exosomal miR-374a-5p inhibited the progression of renal fibrosis by regulating MAPK6/MK5/YAP axis.",
"descriptionType": "Abstract"
}
],
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"relationType": "IsSupplementTo",
"relatedIdentifier": "10.1080/21655979.2022.2033465",
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}
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