{
"id": "https://doi.org/10.6084/m9.figshare.12129408",
"doi": "10.6084/M9.FIGSHARE.12129408",
"url": "https://tandf.figshare.com/articles/figure/MicroRNA-409-5p_promotes_retinal_neovascularization_in_diabetic_retinopathy/12129408",
"types": {
"ris": "FIGURE",
"bibtex": "misc",
"citeproc": "graphic",
"schemaOrg": "ImageObject",
"resourceType": "Figure",
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},
"creators": [
{
"name": "Wang, Ying",
"givenName": "Ying",
"familyName": "Wang"
},
{
"name": "Lin, Wenwen",
"givenName": "Wenwen",
"familyName": "Lin"
},
{
"name": "Ju, Jianghua",
"givenName": "Jianghua",
"familyName": "Ju"
}
],
"titles": [
{
"title": "MicroRNA-409-5p promotes retinal neovascularization in diabetic retinopathy"
}
],
"publisher": {
"name": "Taylor & Francis"
},
"container": {},
"subjects": [
{
"subject": "Medicine"
},
{
"subject": "Cell Biology"
},
{
"subject": "Genetics"
},
{
"subject": "FOS: Biological sciences",
"schemeUri": "http://www.oecd.org/science/inno/38235147.pdf",
"subjectScheme": "Fields of Science and Technology (FOS)"
},
{
"subject": "Molecular Biology"
},
{
"subject": "Chemical Sciences not elsewhere classified"
},
{
"subject": "Biological Sciences not elsewhere classified"
},
{
"subject": "Developmental Biology"
},
{
"subject": "Marine Biology"
},
{
"subject": "Cancer"
}
],
"contributors": [],
"dates": [
{
"date": "2020-04-15",
"dateType": "Created"
},
{
"date": "2023-05-31",
"dateType": "Updated"
},
{
"date": "2020",
"dateType": "Issued"
}
],
"publicationYear": 2020,
"identifiers": [],
"sizes": [
"197108 Bytes"
],
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"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
"schemeUri": "https://spdx.org/licenses/",
"rightsIdentifier": "cc-by-4.0",
"rightsIdentifierScheme": "SPDX"
}
],
"descriptions": [
{
"description": "Background: Retinal neovascularization, which is characterized by the increased proliferation, migration, and tube formation of retinal microvascular endothelial cells (RMECs), contributes to the progression of diabetic retinopathy (DR). MiR-409-5p has been reported to be upregulated in peripheral blood of DR patients and in vascular endothelial growth factor (VEGF)-induced RMECs. However, the role of miR-409-5p in retinal neovascularization of DR remains unelucidated. Method: The expression of miR-409-5p was measured in retinal tissues of streptozocin-induced and db/db diabetic mice, in high glucose-induced mouse RMECs (mRMECs), and in vitreous fluid of proliferative DR patients. Antagomir of miR-409-5p was intravitreally injected into diabetic mice. Proliferation, migration, and tube formation were detected using cell counting kit-8 assay, transwell assay, and microscope observation, respectively. Luciferase reporter assay was used to detect the direct interaction between miR-409-5p and peroxisome proliferator-activated receptor-α (PPARα). Result: MiR-409-5p was upregulated in retinal tissues of diabetic mice, in high glucose-induced mRMECs, and in vitreous fluid of proliferative DR patients. The knockdown of miR-409-5p attenuated retinal neovascularization in vivo. The overexpression of miR-409-5p promotes the proliferation, migration, and tube formation, and increased VEGF expression and secretion, while the knockdown of miR-409-5p suppressed the VEGF-induced retinal neovascularization in vitro. PPARα is a downstream target of miR-409-5p, and PPARα overexpression negated the promotion of miR-409-5p overexpression on the proliferation, migration, and tube formation of mRMECs. Conclusion: Our findings demonstrated that miR-409-5p acted as a neovasculogenic factor in DR, and anti-miR-409-5p therapy may provide a novel strategy in treating DR.",
"descriptionType": "Abstract"
}
],
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"fundingReferences": [],
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"relationType": "IsSupplementTo",
"relatedIdentifier": "10.1080/15384101.2020.1749484",
"relatedIdentifierType": "DOI"
}
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}