{
"id": "https://doi.org/10.25402/fsg.c.4452560.v2",
"doi": "10.25402/FSG.C.4452560.V2",
"url": "https://future-science-group.figshare.com/collections/Supplementary_information_for_Rapid_Identification_of_Genome_Edited_Mesenchymal_Stem_Cell_Colonies_via_Cas9/4452560/2",
"types": {
"ris": "GEN",
"bibtex": "misc",
"citeproc": "article",
"schemaOrg": "Collection",
"resourceType": "Collection",
"resourceTypeGeneral": "Collection"
},
"creators": [
{
"name": "Jiang, Wei",
"givenName": "Wei",
"familyName": "Jiang"
},
{
"name": "Lian, Wei",
"givenName": "Wei",
"familyName": "Lian"
},
{
"name": "Li, Wenlei",
"givenName": "Wenlei",
"familyName": "Li"
},
{
"name": "Huang, Jieyong",
"givenName": "Jieyong",
"familyName": "Huang"
},
{
"name": "Lai, Baoyu",
"givenName": "Baoyu",
"familyName": "Lai"
},
{
"name": "Li, Lingyun",
"givenName": "Lingyun",
"familyName": "Li"
},
{
"name": "Huang, Zhong",
"givenName": "Zhong",
"familyName": "Huang"
},
{
"name": "Xu, Jianyong",
"givenName": "Jianyong",
"familyName": "Xu"
}
],
"titles": [
{
"title": "Supplementary information for: Rapid Identification of Genome Edited Mesenchymal Stem Cell Colonies via Cas9"
}
],
"publisher": {
"name": "Future Science Group"
},
"container": {},
"subjects": [
{
"subject": "Genomics",
"schemeUri": "http://www.abs.gov.au/ausstats/abs@.nsf/0/6BB427AB9696C225CA2574180004463E",
"subjectScheme": "ANZSRC Fields of Research",
"classificationCode": "310509"
}
],
"contributors": [],
"dates": [
{
"date": "2019-05-16",
"dateType": "Created"
},
{
"date": "2019-08-21",
"dateType": "Updated"
},
{
"date": "2019",
"dateType": "Issued"
}
],
"publicationYear": 2019,
"identifiers": [],
"sizes": [],
"formats": [],
"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
"schemeUri": "https://spdx.org/licenses/",
"rightsIdentifier": "cc-by-4.0",
"rightsIdentifierScheme": "SPDX"
}
],
"descriptions": [
{
"description": "Supplementary Figure 1. MSC characterization prior to the genome\n editing. (A) The morphology of the MSCs. (B) The MSCs expressed MSC markers,\n analyzed by flow cytometry. (C) The MSCs could differentiate into adipocytes,\n osteocytes and chondrocytes.\n \n Supplementary Figure 2. Target inserting the IL-37 into AASV1\n site. (A) Donor and gRNA structures. (B) PCR characterization of genome\n edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR\n product size. (C) PCR characterization of genome edited MSC colonies by amplifying\n the right arm. Arrow shows the predicted PCR product size. (D) mRNA level of\n IL-37 among the colony 8 (C8) without donor insertion, colony 20 (C20) with\n correct insertion, and the wild type of MSCs (NC). M: DNA ladder; arrows\n showed the DNA size with 750bp, 1.0kb or 2kb.",
"descriptionType": "Abstract"
}
],
"geoLocations": [],
"fundingReferences": [],
"relatedIdentifiers": [
{
"relationType": "IsIdenticalTo",
"relatedIdentifier": "10.25402/fsg.c.4452560",
"relatedIdentifierType": "DOI"
}
],
"relatedItems": [],
"schemaVersion": "http://datacite.org/schema/kernel-4",
"providerId": "rclg",
"clientId": "figshare.fsg",
"agency": "datacite",
"state": "findable"
}