{
"id": "https://doi.org/10.17044/scilifelab.14730924.v2",
"doi": "10.17044/SCILIFELAB.14730924.V2",
"url": "https://scilifelab.figshare.com/articles/figure/Simultaneous_visualization_of_DNA_loci_in_single_cells_by_combinatorial_multi-color_iFISH_Dataset_9_iFISH_chr10_spotting_Replicate_2_/14730924/2",
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"creators": [
{
"name": "Faustino Mota, Ana",
"givenName": "Ana",
"familyName": "Faustino Mota"
},
{
"name": "Schweitzer, Maud",
"givenName": "Maud",
"familyName": "Schweitzer"
},
{
"name": "Bienko, Magda",
"givenName": "Magda",
"familyName": "Bienko"
}
],
"titles": [
{
"title": "Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH (Dataset 9: iFISH chr10 spotting Replicate 2)"
}
],
"publisher": {
"name": "SciLifeLab"
},
"container": {},
"subjects": [
{
"subject": "60407 Genome Structure and Regulation",
"schemeUri": "http://www.abs.gov.au/ausstats/abs@.nsf/0/6BB427AB9696C225CA2574180004463E",
"subjectScheme": "FOR"
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{
"subject": "FOS: Biological sciences",
"schemeUri": "http://www.oecd.org/science/inno/38235147.pdf",
"subjectScheme": "Fields of Science and Technology (FOS)"
}
],
"contributors": [],
"dates": [
{
"date": "2021-10-25",
"dateType": "Created"
},
{
"date": "2021-10-25",
"dateType": "Updated"
},
{
"date": "2021",
"dateType": "Issued"
}
],
"publicationYear": 2021,
"identifiers": [],
"sizes": [
"12049240443 Bytes"
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"rightsList": [
{
"rights": "Creative Commons Attribution 4.0 International",
"rightsUri": "https://creativecommons.org/licenses/by/4.0/legalcode",
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"descriptions": [
{
"description": "We designed 13 probes targeting 13 consecutive loci on chromosome 10 using iFISH4U. The 23 loci are spaced with 10 Mb. There are 2 probes with AF488 dye, 2 probes with AT542, 3 probes with AF594, 2 probes with AT647N, 2 probes with AF700 and 2 probes with AF790. Replicate 2
Experiment Imaging Method: epifluorescence microscopy
Protocol Name: iFISH protocol
Each dataset consists of multiple fields of view (FOVs) that were acquired with seven different dyes (AF488 labeled as ‘a488’; AT542 as ‘tmr’; AF594 as ‘a594’; AT647N as ‘Cy5’, AF700 as ‘a700’, AF790 as ‘ir800’; and Hoechst 33342 as ‘dapi’) and all the FOVs are provided as separate .tiff files. Each FOV comprises 81–95 focal planes spaced 0.2 μm apart and has a size of 1024x1024 pixels.
",
"descriptionType": "Abstract"
}
],
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