{
"@context": "http://schema.org",
"@type": "ScholarlyArticle",
"@id": "https://doi.org/10.6084/m9.figshare.6073331.v1",
"url": "https://tandf.figshare.com/articles/journal_contribution/Development_and_validation_of_polar_RP-HPLC_method_for_screening_for_ectoine_high-yield_strains_in_marine_bacteria_with_green_chemistry/6073331/1",
"additionalType": "Journal contribution",
"name": "Development and validation of polar RP-HPLC method for screening for ectoine high-yield strains in marine bacteria with green chemistry",
"author": [
{
"name": "Jun Chen",
"givenName": "Jun",
"familyName": "Chen"
},
{
"name": "Jianwei Chen",
"givenName": "Jianwei",
"familyName": "Chen"
},
{
"name": "Sijia Wang",
"givenName": "Sijia",
"familyName": "Wang"
},
{
"name": "Guangmin Zhou",
"givenName": "Guangmin",
"familyName": "Zhou"
},
{
"name": "Danqing Chen",
"givenName": "Danqing",
"familyName": "Chen"
},
{
"name": "Huawei Zhang",
"givenName": "Huawei",
"familyName": "Zhang"
},
{
"name": "Hong Wang",
"givenName": "Hong",
"familyName": "Wang"
}
],
"description": "A novel, green, rapid, and precise polar RP-HPLC method has been successfully developed and screened for ectoine high-yield strain in marine bacteria. Ectoine is a polar and extremely useful solute which allows microorganisms to survive in extreme environmental salinity. This paper describes a polar-HPLC method employed polar RP-C18 (5 μm, 250 × 4.6 mm) using pure water as the mobile phase and a column temperature of 30 °C, coupled with a flow rate at 1.0 mL/min and detected under a UV detector at wavelength of 210 nm. Our method validation demonstrates excellent linearity (R\n2 = 0.9993), accuracy (100.55%), and a limit of detection LOQ and LOD of 0.372 and 0.123 μgmL−1, respectively. These results clearly indicate that the developed polar RP-HPLC method for the separation and determination of ectoine is superior to earlier protocols.",
"license": "https://creativecommons.org/licenses/by/4.0/legalcode",
"keywords": "Biochemistry, Space Science, Environmental Sciences not elsewhere classified, Immunology, FOS: Clinical medicine, Biological Sciences not elsewhere classified, Marine Biology, Inorganic Chemistry, FOS: Chemical sciences",
"contentSize": "161710 Bytes",
"dateCreated": "2018-04-02",
"datePublished": "2018",
"dateModified": "2024-02-16",
"sameAs": {
"@id": "https://doi.org/10.6084/m9.figshare.6073331",
"@type": "CreativeWork"
},
"@reverse": {
"isBasedOn": {
"@id": "https://doi.org/10.1080/14786419.2018.1457668",
"@type": "ScholarlyArticle"
}
},
"schemaVersion": "http://datacite.org/schema/kernel-4",
"publisher": {
"@type": "Organization",
"name": "Taylor & Francis"
},
"provider": {
"@type": "Organization",
"name": "datacite"
}
}